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ACOT1 Antibody (PA5-117764) in ICC/IF
Immunofluorescence staining of ACOT1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ACOT1 Polyclonal Antibody (Product # PA5-117764, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Product Details
PA5-117764
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived Human ACOT1 fragment
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2902375
Target Information
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16. Hunt M.C., Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases. Li W.B., Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acyl-CoA thioesterase 1; Acyl-coenzyme A thioesterase 1; CTE-I; CTE-Ib; Inducible cytosolic acyl-coenzyme A thioester hydrolase; L CTE-I; Long chain acyl-CoA hydrolase; Long chain acyl-CoA thioester hydrolase; Palmitoyl-coenzyme A thioesterase
Gene Aliases: ACH2; ACOT1; CTE-1; CTE1; LACH2
UniProt ID: (Human) Q86TX2
Entrez Gene ID: (Human) 641371
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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