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ACSS2 Antibody (600-401-GW7) in WB
Western Blot of Rabbit anti-ACSS2 antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: HEPG2 WCL. Load: 10 µg per lane. Primary antibody: ACSS2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Block: BlockOut (p/n MB-070) 30 min at RT. Predicted/Observed size: Expect 76 kDa for ACSS2 protein.
Product Details
600-401-GW7
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
ACSS2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic peptide near the N-term of human ACSS2.
Conjugate
Form
Liquid
Concentration
1.01 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Directed against human ACSS2 protein. Blast analysis of the sequence of the immunogen shows 100% identity with human and 80% identity to mouse.
Target Information
This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AceCS1; acetate thiokinase; Acetate--CoA ligase; acetate-CoA ligase; Acetyl-CoA synthetase; Acetyl-CoA synthetase 1; acetyl-Coenzyme A synthetase 2 (ADP forming); Acetyl-coenzyme A synthetase, cytoplasmic; ACS; Acyl-activating enzyme; Acyl-CoA synthetase short-chain family member 2; cytoplasmic acetyl-coenzyme A synthetase; Propionate--CoA ligase
Gene Aliases: ACAS2; ACECS; ACS; ACSA; ACSS2; dJ1161H23.1
UniProt ID: (Human) Q9NR19
Entrez Gene ID: (Human) 55902
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