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产品信息
14-4040-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rat
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
9ACT12
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1723991
产品详细信息
Description: The monoclonal antibody 9ACT12 reacts with human Act1 (NF-kappaB activator 1), also known as CIKS (a connection to IkB kinase and stress-activated kinase). Originally cloned for its ability to activate NF-kappaB and its direct interaction with IKKgamma, Act1 has been subsequently shown to play several roles in immunity. In B cells, it is a negative regulator of CD40 and BAFFR signaling affecting reduced survival. This regulation is through direct interaction of Act1 with CD40 or BAFFR and TRAF3. In addition, Act1 is involved in IL-17R signaling. It interacts with IL-17RA through the homotypic SEFIR interaction domain and is critical for downstream NF-kappaB- and C/EBP-mediated inflammatory responses.
Applications Reported: This 9ACT12 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This 9ACT12 antibody has been tested by immunoblot of human peripheral blood cell lysates. This can be used at less than or equal to 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This gene encodes a protein involved in regulating responses to cytokines by members of the Rel/NF-kappaB transcription factor family. These factors play a central role in innate immunity in response to pathogens, inflammatory signals and stress. This gene product interacts with TRAF proteins (tumor necrosis factor receptor-associated factors) and either I-kappaB kinase or MAP kinase to activate either NF-kappaB or Jun kinase. Two alternative transcripts encoding different proteins have been identified. A third transcript, which does not encode a protein and is transcribed in the opposite orientation, has been identified. Overexpression of this transcript has been shown to reduce expression of at least one of the protein encoding transcripts, suggesting it has a regulatory role in the expression of this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ACT1; Adapter protein CIKS; Connection to IKK and SAPK/JNK; E3 ubiquitin ligase TRAF3IP2; E3 ubiquitin-protein ligase CIKS; NFkB-activating protein ACT1; Nuclear factor NF-kappa-B activator 1; RP3-487J7.3; TRAF3-interacting protein 2
基因别名: ACT1; C6orf2; C6orf4; C6orf5; C6orf6; CANDF8; CIKS; PSORS13; TRAF3IP2
UniProt ID: (Human) O43734
Entrez Gene ID: (Human) 10758
