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ADH4 Antibody (PA5-95375) in WB
Western blot analysis of ADH4 in rat liver extract (lane 1), mouse liver extract (lane 2) and HEPG2 whole cell lysates (lane 3). Samples were incubated with ADH4 polyclonal antibody (Product # PA5-95375) using a 0.5 µg/mL dilution. developed was performed using enhanced chemiluminescence (ECL).
Product Details
PA5-95375
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ADH4 recombinant protein (Position: K218-F380).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807178
Product Specific Information
Human ADH4 shares 68.7% and 71.8% amino acid (aa) sequence identity with mouse and rat ADH4, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat liver tissue, mouse liver tissueHEPG2 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADH2; Alcohol dehydrogenase 2; Alcohol dehydrogenase 4; Alcohol dehydrogenase class II; Alcohol dehydrogenase class II pi chain; Alcohol dehydrogenase II; aldehyde reductase; All-trans-retinol dehydrogenase [NAD(+)] ADH4; class II alcohol dehydrogenase, pi subunit; class II type ADH; epididymis secretory protein Li 4
Gene Aliases: Ac1002; ADH-1; ADH-2; ADH2; ADH4; HEL-S-4
UniProt ID: (Human) P08319, (Mouse) Q9QYY9
Entrez Gene ID: (Human) 127, (Mouse) 26876, (Rat) 29646
NADPH:quinone reductase activity
alcohol dehydrogenase (NAD) activity
alcohol dehydrogenase activity, zinc-dependent
alditol:NADP+ 1-oxidoreductase activity
retinol dehydrogenase activity
all-trans retinal binding
zinc ion binding
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
benzaldehyde dehydrogenase activity
retinol binding
ethanol binding
NAD binding
oxidoreductase activity
metal ion binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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