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AGO1 Antibody (702720) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), NTERA-2 cl.D1 (Lane 2), HEL 92.1.7 (Lane 3), K-562 (Lane 4), MOLT4 (Lane 5), HL-60 (Lane 6), A-431 (Lane 7) and THP-1 (Lane 8). The blots were probed with Anti-AGO1 Recombinant Rabbit Monoclonal Antibody (Product # 702720, 1 µg/mL). A 97 kDa band corresponding to AGO1 was observed across the cell lines tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Produc... View More
产品信息
702720
产品规格
种属反应
Human
已发表种属
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
6H1L4
抗原
Peptides corresponding to human AGO1 (1) aa17-aa35 2) aa382-aa401)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2692255
产品详细信息
This antibody is predicted to react with Monkey, Pig, and Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
AGO1 is a member of the Argonaute family of proteins which play a role in RNA interference. The protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. AGO1 binds to miRNAs or siRNAs and represses the translation of mRNAs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: argonaute 1; Argonaute RISC catalytic component 1; Argonaute1; eIF-2C 1; eIF2C 1; Eukaryotic translation initiation factor 2C 1; eukaryotic translation initiation factor 2C, 1; Golgi Endoplasmic Reticulum protein 95 kDa; Protein argonaute-1; Putative RNA-binding protein Q99
基因别名: AGO1; EIF2C; EIF2C1; GERP95; hAgo1; Q99
UniProt ID: (Human) Q9UL18
Entrez Gene ID: (Human) 26523
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core binding
core promoter binding
RNA binding
double-stranded RNA binding
single-stranded RNA binding
endoribonuclease activity
protein binding
miRNA binding
poly(A) RNA binding
translation initiation factor
nuclear-transcribed mRNA catabolic process
transcription, DNA-templated
Wnt signaling pathway, calcium modulating pathway
RNA secondary structure unwinding
miRNA metabolic process
gene silencing by RNA
pre-miRNA processing
posttranscriptional gene silencing by RNA
production of miRNAs involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA cleavage involved in gene silencing by miRNA
miRNA loading onto RISC involved in gene silencing by miRNA
positive regulation of transcription from RNA polymerase II promoter
phosphatidylinositol-mediated signaling
mRNA cleavage involved in gene silencing by siRNA
