Invitrogen

AGR2 Monoclonal Antibody (10E2)

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AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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AGR2 Antibody (MA5-16244) in IHC (P)

Immunohistochemical analysis of AGR2 in a human ovarian cancer section. Samples were incubated in AGR2 monoclonal antibody (Product # MA5-16244) using a dilution of 5 µg/mL. The representative image shows AGR2 immuno-reactivity in the developing ovarian cancer and the follicle cells of Graafian follicles. The cells of medulla region in the ovary did not show AGR2 positivity. [10X magnification].

AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AGR2 Antibody in Western Blot (WB)

AGR2 Antibody in Immunohistochemistry (IHC)

AGR2 Antibody in Western Blot (WB)

AGR2 Antibody in Western Blot (WB)

AGR2 Antibody in Western Blot (WB)

Product Image Gallery

AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of AGR2 in a human ovarian cancer section. Samples were incubated in AGR2 monoclonal antibody (Product # MA5-16244) using a dilution of 5 µg/mL. The representative image shows AGR2 immuno-reactivity in the developing ovarian cancer and the follicle cells of Graafian follicles. The cells of medulla region in the ovary did not show AGR2 positivity. [10X magnification].

AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of AGR2 in paraffin-embedded human small intestine tissue. Samples were incubated in AGR2 monoclonal antibody (Product # MA5-16244) using a dilution of 10 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of AGR2 in formalin-fixed, paraffin-embedded human breast. Samples were incubated in AGR2 monoclonal antibody (Product # MA5-16244) using a dilution of 5 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of AGR2 in formalin-fixed, paraffin-embedded human spleen tissue. Samples were incubated in AGR2 monoclonal antibody (Product # MA5-16244) using a dilution of 5 µg/mL.

AGR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of AGR2 in a section of human bladded cancer. Samples were incubated in AGR2 monoclonal antibody (Product # MA5-16244) using a dilution of 5 µg/mL. The AGR2 immunoreactivity was observed in cellular cytoplasm and the inter-cellular spaces in cancerous areas of the sections. [40X magnification].

AGR2 Antibody in Western Blot (WB) — Western blot analysis of AGR2 in human stomach. Samples were incubated in AGR2 monoclonal antibody (Product # MA5-16244) using a dilution of 5 µg/mL followed by a goat anti-mouse Ig HRP secondary antibody. A) absense and B) presence of immunizing peptide, C) mouse stomach, D) rat stomach tissue lysate, and E) HCT-116 cell lysate. PicoTect ECL substrate solution was used for this test.

AGR2 Antibody in Immunohistochemistry (IHC) — Figure 1 AGR2 overexpression in canine MMT tissues revealed by immunohistochemistry (IHC). AGR2 protein expression was analyzed with tissue slides of 34 canine MMT cases using an antibody specific to AGR2. ( A ) Micrographs of two representative cases. Upper micrographs were acquired at 100x magnification; bottom micrographs were the areas in the upper images acquired at 400x magnification. ( B ) MMT tissues stained with an isotype control IgG. ( C ) Pairwise comparison of IHC scores of AGR2 between MMT tissue and adjacent normal mammary gland tissue of the same patient. Each line represents an individual patient. Statistical significance was determined by two-tailed paired t -test. ( D ) Comparison of IHC scores of AGR2 between overall MMT tissues and adjacent normal mammary tissues. L, M, and H denote low, moderate, and high levels of AGR2, respectively. Statistical significance was determined by Fisher's exact test. ** p < 0.01; *** p < 0.001.

AGR2 Antibody in Western Blot (WB) — Figure 2 Elevated protein levels of AGR2 in canine MMT tissues compared with paired normal mammary tissues. Tissue homogenates (20 ug proteins each sample) of MMT (denoted M), paired with benign mammary tumor (denoted B) and normal mammary gland tissues (denoted N) collected from the same patient, were analyzed by immunoblotting using an antibody specific to AGR2. GAPDH was used as an internal control. Representative results of nine cases (No. 1 to No. 9) were shown in ( A ). AGR2 protein levels in individual samples were determined by calculating the AGR2-to-GAPDH ratio. ( B ) Comparison of AGR2 protein levels between tissue types. Statistical significance was determined by Mann-Whitney U test. ( C ) Pairwise comparison of AGR2 protein levels between MMT and paired normal mammary gland tissues of the same patient. Lines represent individual patients. Statistical significance was determined by two-tailed paired t -test. ** p < 0.01; *** p < 0.001.

AGR2 Antibody in Western Blot (WB) — Figure 4 Establishment of a competitive ELISA for detection of serum eAGR2 in MMT dogs. ( A ) Scheme for the established competitive ELISA. ( B ) Identity of recombinant canine AGR2 (rcAGR2) was confirmed by immunoblotting using antibodies specific to AGR2 and 6xHis tag, respectively. ( C ) A standard curve with fourfold serially diluted rcAGR2 proteins (8000, 2000, 500, 125, and 31.25 ng/mL) was set for the competitive ELISA. Data are presented as the mean values +- SD across 10 assays. ( D ) Precision of the competitive ELISA. The intra-assay coefficient variant (CV), inter-assay CV, and the detection sensitivity were determined across 10 assays. ( E ) Evaluation of serum influence on eAGR2 detection in the ELISA. A spike recovery test was performed by detecting rcAGR2 spiked at indicated concentrations into canine serum containing undetectable eAGR2. The recovery rate (%) of individual spike rcAGR2 was calculated as indicated.

AGR2 Antibody in Western Blot (WB) — Figure 3 Detection of AGR2 expression in canine MMT cells and conditioned media. ( A ) Intracellular localization of AGR2 in canine MMT cell lines, DMGT, and CF41.Mg. AGR2 expression was verified by immunofluorescence staining using an AGR2-specific antibody. DAPI staining indicated the nuclei. Magnification, x40. ( B ) Detection of AGR2 in cell lysates of canine MMT cell lines. Cell lysates (30 ug proteins per sample) were analyzed by immunoblotting with an AGR2-specific antibody. ( C ) Detection of extracellular AGR2 (eAGR2) in serum-free conditioned media of canine MMT cells. Cells were grown in serum-free media for one day, and the conditioned media were harvested and subsequently concentrated. The resulting conditioned media (50 ug proteins per sample) were analyzed using a competitive ELISA established for eAGR2 detection.

产品信息

MA5-16244

应用

建议稀释比

已发表文章

免疫印迹 (WB)

4-6 µg/mL

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

5 µg/mL

-

免疫组化（冰冻） (IHC (F))

-

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免疫细胞化学 (ICC/IF)

-

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产品规格

种属反应

Bovine, Dog, Horse, Human, Mouse, Non-human primate, Rat

已发表种属

Dog

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

10E2

抗原

A portion of amino acids 50-100 of human AGR2 was used as the immunogen for the antibody.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2537762

靶标信息

AGR2 (anterior gradient protein 2) and AGR3 are members of the anterior gradient homolog family. They are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours. AGR2 is ubiquitously expressed with upregulated expression in prostate cancer, breast cancer, lung cancer, renal carcinomas and endometrial carcinomas. AGR2 expression is positively correlated with that of the estrogen receptor (ER) and is negatively correlated with that of the epidermal growth factor receptor (EGFR).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Serum Level of Tumor-Overexpressed AGR2 Is Significantly Associated with Unfavorable Prognosis of Canine Malignant Mammary Tumors.

Animals : an open access journal from MDPI

Yuan SH,Chang SC,Huang Y,Liu HP

MA5-16244 was used in Immunocytochemistry-immunoflourescence to validate overexpression of anterior gradient protein 2 (AGR2) in canine malignant mammary tumors (MMTs) tissues from 45 dogs using immunohistochemistry and immunoblotting, and assessed serum AGR2 levels in 81 dogs with MMTs and 21 benign cases using a competitive enzyme-linked immunosorbent assay (ELISA).

Sat Oct 09 00:00:00 UTC 2021

Serum Level of Tumor-Overexpressed AGR2 Is Significantly Associated with Unfavorable Prognosis of Canine Malignant Mammary Tumors.

Animals : an open access journal from MDPI

Yuan SH,Chang SC,Huang Y,Liu HP

MA5-16244 was used in Immunocytochemistry-immunoflourescence to validate overexpression of anterior gradient protein 2 (AGR2) in canine malignant mammary tumors (MMTs) tissues from 45 dogs using immunohistochemistry and immunoblotting, and assessed serum AGR2 levels in 81 dogs with MMTs and 21 benign cases using a competitive enzyme-linked immunosorbent assay (ELISA).

Sat Oct 09 00:00:00 UTC 2021

Serum Level of Tumor-Overexpressed AGR2 Is Significantly Associated with Unfavorable Prognosis of Canine Malignant Mammary Tumors.

Animals : an open access journal from MDPI

Yuan SH,Chang SC,Huang Y,Liu HP

MA5-16244 was used in Immunocytochemistry-immunoflourescence to validate overexpression of anterior gradient protein 2 (AGR2) in canine malignant mammary tumors (MMTs) tissues from 45 dogs using immunohistochemistry and immunoblotting, and assessed serum AGR2 levels in 81 dogs with MMTs and 21 benign cases using a competitive enzyme-linked immunosorbent assay (ELISA).

Sat Oct 09 00:00:00 UTC 2021

Serum Level of Tumor-Overexpressed AGR2 Is Significantly Associated with Unfavorable Prognosis of Canine Malignant Mammary Tumors.

Animals : an open access journal from MDPI

Yuan SH,Chang SC,Huang Y,Liu HP

MA5-16244 was used in Immunocytochemistry-immunoflourescence to validate overexpression of anterior gradient protein 2 (AGR2) in canine malignant mammary tumors (MMTs) tissues from 45 dogs using immunohistochemistry and immunoblotting, and assessed serum AGR2 levels in 81 dogs with MMTs and 21 benign cases using a competitive enzyme-linked immunosorbent assay (ELISA).

Sat Oct 09 00:00:00 UTC 2021

Serum Level of Tumor-Overexpressed AGR2 Is Significantly Associated with Unfavorable Prognosis of Canine Malignant Mammary Tumors.

Animals : an open access journal from MDPI

Yuan SH,Chang SC,Huang Y,Liu HP

MA5-16244 was used in Immunocytochemistry-immunoflourescence to validate overexpression of anterior gradient protein 2 (AGR2) in canine malignant mammary tumors (MMTs) tissues from 45 dogs using immunohistochemistry and immunoblotting, and assessed serum AGR2 levels in 81 dogs with MMTs and 21 benign cases using a competitive enzyme-linked immunosorbent assay (ELISA).

Sat Oct 09 00:00:00 UTC 2021

生物信息学

蛋白别名: AG-2; anterior gradient 2 homolog; anterior gradient 2 homologue; anterior gradient homolog 2; Anterior gradient protein 2 homolog; epididymis secretory protein Li 116; HPC8; protein disulfide isomerase family A, member 17; Protein Gob-4; secreted cement gland homolog; Secreted cement gland protein XAG-2 homolog

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基因别名: AG2; AGR2; Agr2h; BOS_4050; GOB-4; Gob4; HAG-2; HEL-S-116; mAG-2; PDIA17; UNQ515/PRO1030; XAG-2

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UniProt ID: (Human) O95994, (Mouse) O88312

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Entrez Gene ID: (Human) 10551, (Bovine) 415112, (Dog) 482333, (Mouse) 23795, (Rat) 298961

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dystroglycan binding epidermal growth factor receptor binding protein binding protein homodimerization activity molecular_function

positive regulation of gene expression positive regulation of cell-substrate adhesion positive regulation of epidermal growth factor receptor signaling pathway lung goblet cell differentiation negative regulation of cell death mucus secretion positive regulation of establishment of protein localization to plasma membrane positive regulation of IRE1-mediated unfolded protein response positive regulation of PERK-mediated unfolded protein response response to endoplasmic reticulum stress digestive tract morphogenesis positive regulation of developmental growth biological_process