Invitrogen

AHNAK Polyclonal Antibody

AHNAK Antibody in Immunocytochemistry (ICC/IF)

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AHNAK Antibody (PA5-53890) in ICC/IF

Immunofluorescence analysis of AHNAK was performed using 70% confluent log phase HaCaT cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with AHNAK Polyclonal Antibody (Product # PA5-53890, 0.25 µg/mL) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:20... View More

AHNAK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AHNAK Antibody in Immunohistochemistry (IHC)

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AHNAK Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of AHNAK was observed in U-87 MG, PANC-1, 769-P, NIH: OVCAR-3, HaCaT which are high expressing cell lines and was not observed in Jurkat, A-673, HEL 92.1.7 which are low expressing cell lines using AHNAK Polyclonal Antibody (Product # PA5-53890) in Western Blot. {RE}

产品信息

PA5-53890

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20-1:50

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human AHNAK. Recombinant protein control fragment (Product #RP-92506).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2637783

产品详细信息

Immunogen sequence: LGEGHLSVKG SGGEWKGPQV SSALNLDTSK FAGGLHFSGP KVEGGVKGGQ IGLQAPGLSV SGPQGHLESG SGKVTFPKMK IPKFTFSGRE LVGREMGVDV HFPKAEASIQ AGAGDGEWEE SEVKLKKSKI KMPK

Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 84%.

靶标信息

AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。