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Invitrogen
AHNAK Recombinant Rabbit Monoclonal Antibody (PSH01-10)
FIGURE: 1 / 17
AHNAK Antibody (MA5-49610) in ICC/IF
Immunofluorescence analysis of AHNAK was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with AHNAK Rabbit Recombinant Monoclonal Antibody (PSH01-10) (Product # MA5-49610) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa F... View More
Product Details
MA5-49610
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH01-10
Immunogen
Recombinant protein within human AHNAK aa 451-900/5,890.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3092422
Product Specific Information
Sequence Similarities: 100% Mouse/Rat.
Tissue Specificity: Low tissue specificity.
Positive Control: Human lung carcinoma tissue, human brain tissue, mouse brain tissue, rat brain tissue, A431, HeLa, NIH/3T3.
Subcellular Location: Nucleus.
Predicted band size: 629 kDa.
Target Information
AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AHNAK-related; Desmoyokin; MGC5395; Neuroblast differentiation-associated protein AHNAK
Gene Aliases: AHNAK; AHNAK 1; AHNAKRS; DY6; PM227; RGD1308572; UV118
UniProt ID: (Human) Q09666
Entrez Gene ID: (Human) 79026, (Rat) 191572, (Mouse) 66395
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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