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AIF Antibody (MA5-15880) in ICC/IF
Immunofluorescent analysis of Cytochrome C (red) in HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes at -20°C, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Cytochrome C monoclonal antibody, Alexa Fluor 647 conjugate (Product # MA3-38200-A647) at a dilution of 10 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Cells were then stained with a AIF mouse monoclonal antibody (Product # MA5-15880) at ... View More
产品信息
MA5-15880
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
4E7E11
抗原
Purified recombinant fragment of human AIF expressed in E. Coli.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_11153781
产品详细信息
MA5-15880 targets AIF in FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
The MA5-15880 immunogen is purified recombinant fragment of human AIF expressed in E. Coli. .
MA5-15880 detects AIF which has a predicted molecular weight of approximately 67kDa.
靶标信息
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: AG1; AGR1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; ER protein 18; ER protein 19; ERP18; ERP19; hAG-1; harlequin; hTLP19; MGC111425; pcd8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376
基因别名: AIF; AIFM1; AIFsh2; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; Hq; NADMR; NAMSD; PDCD8
UniProt ID: (Human) O95831, (Mouse) Q9Z0X1, (Rat) Q9JM53
Entrez Gene ID: (Human) 9131, (Mouse) 26926, (Rat) 83533
DNA binding
protein binding
electron carrier activity
NAD(P)H oxidase activity
oxidoreductase activity, acting on NAD(P)H
protein dimerization activity
FAD binding
C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity
aldo-keto reductase (NADP) activity
electron-transferring-flavoprotein dehydrogenase activity
isocitrate dehydrogenase activity
mevaldate reductase activity
gluconate dehydrogenase activity
steroid dehydrogenase activity
oxidoreductase activity
epoxide dehydrogenase activity
5-exo-hydroxycamphor dehydrogenase activity
2-hydroxytetrahydrofuran dehydrogenase activity
acetoin dehydrogenase activity
phenylcoumaran benzylic ether reductase activity
D-xylose:NADP reductase activity
L-arabinose:NADP reductase activity
D-arabinitol dehydrogenase, D-ribulose forming (NADP+) activity
steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
steroid dehydrogenase activity, acting on the CH-CH group of donors
(R)-(-)-1,2,3,4-tetrahydronaphthol dehydrogenase activity
3-hydroxymenthone dehydrogenase activity
very long-chain-3-hydroxyacyl-CoA dehydrogenase activity
dihydrotestosterone 17-beta-dehydrogenase activity
(R)-2-hydroxyisocaproate dehydrogenase activity
L-arabinose 1-dehydrogenase (NADP+) activity
L-xylulose reductase (NAD+) activity
3-ketoglucose-reductase activity
flavin adenine dinucleotide binding
(R)-2-hydroxyglutarate dehydrogenase activity
D-arabinitol dehydrogenase, D-xylulose forming (NADP+) activity
response to ischemia
DNA catabolic process
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to toxic substance
chromosome condensation
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
cell redox homeostasis
neuron apoptotic process
oxidation-reduction process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hydrogen peroxide
cellular response to estradiol stimulus
cellular response to nitric oxide
cellular response to oxygen-glucose deprivation
response to L-glutamate
regulation of apoptotic DNA fragmentation
cellular response to aldosterone
response to oxidative stress
apoptotic mitochondrial changes
positive regulation of cell death
neuron differentiation
mitochondrial respiratory chain complex I assembly
