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AKAP11 Antibody (PA5-103369) in ICC/IF
Immunocytochemistry analysis of AKAP11 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with AKAP11 polyclonal antibody (Product # PA5-103369) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-103369
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human AKAP11(Accession Q9UKA4), corresponding to amino acid residues N1761-L1811.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2852713
产品详细信息
Antibody detects endogenous levels of total AKAP11.
靶标信息
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed at high levels throughout spermatogenesis and in mature sperm. It binds the RI and RII subunits of PKA in testis. It may serve a function in cell cycle control of both somatic cells and germ cells in addition to its putative role in spermatogenesis and sperm function. [provided by RefSeq, Jul 2008].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: A kinase (PRKA) anchor protein 11; a kinase anchor protein 220 kDa; A-kinase anchor protein 11; A-kinase anchor protein 220 kDa; A-kinase anchoring protein, 220kDa; AKAP 220; AKAP-11; PRKA11; protein kinase A anchoring protein 11; Protein kinase A-anchoring protein 11; protein phosphatase 1, regulatory subunit 44
基因别名: AKAP-11; AKAP11; AKAP220; KIAA0629; PPP1R44; PRKA11
UniProt ID: (Human) Q9UKA4
Entrez Gene ID: (Human) 11215
