Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
AKAP150 Antibody (APZ-031-200UL) in WB
Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat dorsal root ganglion lysates (lanes 3 and 7) and mouse lung lysates (lanes 4 and 8): - 1-4. Anti-AKAP150 Antibody (#APZ-031), (1:200).5-8. Anti-AKAP150 Antibody , preincubated with AKAP150 Blocking Peptide (#BLP-PZ031).
产品信息
APZ-031-200UL
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
(C)RGAKRSRPSKLTED, corresponding to amino acid residues 128-141 of rat AKAP5, Intracellular
偶联物
形式
Lyophilized
浓度
0.8 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 1% BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
靶标信息
AKAPs (A-kinase anchoring proteins), as their name implies, are a family of scaffolding proteins that bind regulatory subunits of Protein Kinase A (PKA) thus localizing PKA activity to distinct regions of the cell. Beyond a common amphipathic alpha helix that is responsible for recruiting the PKA regulatory subunit (RI alpha, RII alpha, RI beta, or RII beta), individual AKAPs contain additional domains responsible for the recrutiment of additional signaling proteins (phosphodiesterases, phosphatases, cytoskeletal components, other kinase, etc.) or restricting AKAP to a specific subcellular location. AKAP5 (also known as P75, AKAP75, or AKAP79) is predominantly expressed in neuronal tissues and cells where it serves to localize type II PKA to post-synaptic densities. AKAP5 specifically binds to the regulatory subunit of PKAII beta, anchoring the enzyme to the plasma membrane and sites of cytoskeletal/membrane junctions. The other binding domains of AKAP5 have been shown to interact with calmodulin, PP2B, and calcineurin suggesting that AKAP5 may act to coordinate the cAMP- and Ca2+-sensing pathways in various cell types.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: A-kinase anchor protein 150 kDa; A-kinase anchor protein 5; AKAP 150; AKAP 5; AKAP-5; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein; RII-B-binding protein
基因别名: 3526401B18Rik; AKAP 150; AKAP-5; AKAP150; Akap5; Akap79; BB098886; Gm258; P150
UniProt ID: (Mouse) D3YVF0
Entrez Gene ID: (Mouse) 238276, (Rat) 171026
G-protein coupled receptor binding
actin binding
protein binding
calmodulin binding
adenylate cyclase binding
kinase binding
protein kinase binding
protein domain specific binding
protein phosphatase 2B binding
protein complex binding
protein kinase A regulatory subunit binding
cadherin binding
GABA receptor binding
scaffold/adaptor protein
positive regulation of protein phosphorylation
protein targeting
signal transduction
negative regulation of adenylate cyclase activity
positive regulation of calcium ion transport into cytosol
positive regulation of protein kinase A signaling
positive regulation of cAMP biosynthetic process
amylase secretion
negative regulation of potassium ion transport
negative regulation of ion transport
cellular protein complex disassembly
positive regulation of adenylate cyclase activity
positive regulation of dendrite morphogenesis
positive regulation of NFAT protein import into nucleus
response to muscle activity
spinal cord development
hippocampus development
response to electrical stimulus
maternal process involved in female pregnancy
cellular response to organonitrogen compound
