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ALAS1 Antibody (PA5-100995) in ICC/IF
Immunofluorescent analysis of ALAS1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ALAS1 polyclonal antibody (Product # PA5-100995) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-100995
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ALAS1(Accession P13196), corresponding to amino acid residues C398-G448.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2850475
产品详细信息
Antibody detects endogenous levels of total Alas1.
靶标信息
ALAS-1 is a nuclear-encoded mitochondrial matrix enzyme which catalyzes the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 5-aminolevulinate synthase, non-specific, mitochondrial; 5-aminolevulinate synthase, nonspecific, mitochondrial; 5-aminolevulinic acid synthase 1; ALAS-H; aminolevulinate synthase H; aminolevulinate, delta-, synthase 1; Delta-ALA synthase 1; Delta-aminolevulinate synthase 1; migration-inducing protein 4; succinyl-CoA: glycine C-succinyl transferase
基因别名: ALAS; Alas-1; ALAS-H; ALAS-N; ALAS1; ALAS3; ALASH; MIG4; OK/SW-cl.121
UniProt ID: (Human) P13196, (Mouse) Q8VC19
Entrez Gene ID: (Human) 211, (Mouse) 11655
