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ALDH1L1 Antibody (13-9595-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human cerebellum using 20 µg/mL Mouse IgG2b K Isotype Control Biotin (left) or 20 µg/mL Anti-Human 10-formyltetrahydrofolate dehydrogenase (ALDH1L1) Biotin (right) followed by streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.
产品信息
13-9595-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
7G8
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572814
产品详细信息
Description: This 7G8 monoclonal antibody reacts with human 10-formyltetrahydrofolate dehydrogenase (ALDH1L1) a protein involved in folate metabolism. The cytosolic enzyme, with homology to the aldehyde dehydrogenase (ALDH) family, is involved in the conversion of 10-formyltetrahydrofolate, NADP and water to tetrahydrofolate, NADPH and carbon dioxide. Folate metabolism is essential for cell growth and proliferation and decreased levels of folate have been implicated in neural tube defects in addition to increased cancer risk. This isoform (1L1) is expressed in glial cells has been identified as a highly specific marker for astrocytes labeling both the cell body and processes.
Applications Reported: This 7G8 antibody has been reported for use in flow cytometric analysis, western blotting, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This 7G8 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue with low pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 10-formyltetrahydrofolate dehydrogenase; 10-FTHFDH; 10fTHF; 10FTHFDH; aldehyde dehydrogenase 1 family, member L1; Aldehyde dehydrogenase family 1 member L1; Cytosolic 10-formyltetrahydrofolate dehydrogenase; DKFZp781N0997; MER57B-ALDH1L1
基因别名: 10-fTHF; 10-FTHFDH; ALDH1L1; FDH; FTHFD
UniProt ID: (Human) O75891
Entrez Gene ID: (Human) 10840
catalytic activity
aldehyde dehydrogenase (NAD) activity
formyltetrahydrofolate dehydrogenase activity
hydroxymethyl-, formyl- and related transferase activity
aminobutyraldehyde dehydrogenase activity
4-trimethylammoniobutyraldehyde dehydrogenase activity
dehydrogenase
RNA processing factor
oxidoreductase
metabolite interconversion enzyme
