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ALG1 Antibody (PA1-031) in WB
Western blot analysis of Alg-1 was performed by loading 15 µg WT or Alg-1 (-) worm extract onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing Alg-1 (Product # PA1-031) at a dilution of 1:5000 for 1 hour and room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution o... View More
Product Details
PA1-031
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
C. elegans
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide sequence DLEEIPNSPPTQPQTFSDVPQR
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539852
Target Information
The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Argonaute; Argonaute-like
Entrez Gene ID: (C. elegans) 181504
nematode larval development
embryo development ending in birth or egg hatching
gene silencing by RNA
production of miRNAs involved in gene silencing by miRNA
mRNA cleavage involved in gene silencing by miRNA
vulval development
regulation of development, heterochronic
hermaphrodite genitalia development
regulation of cell fate specification
small RNA loading onto RISC
RNA phosphodiester bond hydrolysis, endonucleolytic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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