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ALKBH5 Antibody (MA5-51412) in IHC (P)
Immunohistochemistry analysis of ALKBH5 in paraffin-embedded Human kidney. Samples were incubated with ALKBH5 Recombinant monoclonal antibody (Product # MA5-51412) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
MA5-51412
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2S4Q6
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 66-394 of human ALKBH5 (NP_060228.3).
Conjugate
Form
Liquid
Concentration
0.08 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3094255
Product Specific Information
Immunogen sequence: PERSDYEEQQ LQKEEEARKV KSGIRQMRLF SQDECAKIEA RIDEVVSRAE KGLYNEHTVD RAPLRNKYFF GEGYTYGAQL QKRGPGQERL YPPGDVDEIP EWVHQLVIQK LVEHRVIPEG FVNSAVINDY QPGGCIVSHV DPIHIFERPI VSVSFFSDSA LCFGCKFQFK PIRVSEPVLS LPVRRGSVTV LSGYAADEIT HCIRPQDIKE RRAVIILRKT RLDAPRLETK SLSSSVLPPS YASDRLSGNN RDPALKPKRS HRKADPDAAH RPRILEMDKE ENRRSVLLPT HRRRGSFSSE NYWRKSYESS EDCSEAAGSP ARKVKMRRH
Target Information
ALKBH5 (RNA demehtylase ALKBH5) is a dioxygenase that demethylates RNA by oxidative demethylation. Specifically, it demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Demethylation of m6A mRNA affects mRNA processing and export. ALKBH5 can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). It requires molecular oxygen, alpha-ketoglutarate and iron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AlkB family member 5, RNA demethylase; alkB, alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; oxoglutarate and iron-dependent oxygenase domain containing; probable alpha-ketoglutarate-dependent dioxygenase ABH5; RNA demethylase ALKBH5
Gene Aliases: ABH5; ALKBH5; AW050020; AW215868; E130207K11; OFOXD; OFOXD1; RGD1309496
UniProt ID: (Human) Q6P6C2, (Mouse) Q3TSG4, (Rat) D3ZKD3
Entrez Gene ID: (Human) 54890, (Mouse) 268420, (Rat) 303193
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
oxidative RNA demethylase activity
poly(A) RNA binding
metal ion binding
sulfonate dioxygenase activity
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
dioxygenase activity
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
RNA processing factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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