Invitrogen

ALOX15B Polyclonal Antibody

ALOX15B Antibody in Immunocytochemistry (ICC/IF)

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ALOX15B Antibody (PA5-97456) in ICC/IF

Immunofluorescent analysis of ALOX15B in A549 cells using a ALOX15B polyclonal antibody (Product # PA5-97456) at a dilution of 1:166. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

ALOX15B Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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产品信息

PA5-97456

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Arachidonate 15-lipoxygenase B protein (1-676AA)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

4 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

AB_2812073

靶标信息

ALOX15B encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Paradoxical Induction of ALOX15/15B by Cortisol in Human Amnion Fibroblasts: Implications for Inflammatory Responses of the Fetal Membranes at Parturition.

International journal of molecular sciences

Zhang F,Lu JW,Lei WJ,Li MD,Pan F,Lin YK,Wang WS,Sun K

PA5-97456 was used in Western Blot to examine whether glucocorticoids could induce ALOX15/15B expression and 15(S)-HETE production to promote PGE2 synthesis in amnion fibroblasts at parturition.

Thu Jun 29 00:00:00 UTC 2023

Paradoxical Induction of ALOX15/15B by Cortisol in Human Amnion Fibroblasts: Implications for Inflammatory Responses of the Fetal Membranes at Parturition.

International journal of molecular sciences

Zhang F,Lu JW,Lei WJ,Li MD,Pan F,Lin YK,Wang WS,Sun K

PA5-97456 was used in Western Blot to examine whether glucocorticoids could induce ALOX15/15B expression and 15(S)-HETE production to promote PGE2 synthesis in amnion fibroblasts at parturition.

Thu Jun 29 00:00:00 UTC 2023

Identification of a Feed-Forward Loop Between 15(S)-HETE and PGE2 in Human Amnion at Parturition.

Journal of lipid research

Zhang F,Sun K,Wang WS

PA5-97456 was used in Western Blotting to conclude that 15(S)-HETE is identified as another crucial parturition-pertinent AA-derived eicosanoid in the amnion, which may form a feed-forward loop with PGE2 in parturition.

Tue Nov 01 00:00:00 UTC 2022

Identification of a Feed-Forward Loop Between 15(S)-HETE and PGE2 in Human Amnion at Parturition.

Journal of lipid research

Zhang F,Sun K,Wang WS

Tue Nov 01 00:00:00 UTC 2022

Identification of a Feed-Forward Loop Between 15(S)-HETE and PGE2 in Human Amnion at Parturition.

Journal of lipid research

Zhang F,Sun K,Wang WS

Tue Nov 01 00:00:00 UTC 2022

生物信息学

蛋白别名: 15-lipoxygenase 2; 15-LOX-2; 15-LOX-B; 15S-lipoxygenase; arachidonate 15-lipoxygenase 2; Arachidonate 15-lipoxygenase B; Arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type; arachidonate omega(6) lipoxygenase; Linoleate 13-lipoxygenase 15-LOb; Polyunsaturated fatty acid lipoxygenase ALOX15B

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基因别名: 15-LOX-2; ALOX15B

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UniProt ID: (Human) D3DTR2

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Entrez Gene ID: (Human) 247

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功能

iron ion binding calcium ion binding lipid binding linoleate 13S-lipoxygenase activity arachidonate 8(S)-lipoxygenase activity arachidonate 15-lipoxygenase activity oxygenase

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