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ALPL Antibody (MA5-42414) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of Alkaline Phosphatase in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with ALPL Recombinant Monoclonal Antibody (Product # MA5-42414) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nucl... View More
产品信息
MA5-42414
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SA40-00
抗原
Synthetic peptide within human Alkaline Phosphatase aa 18-50
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2911555
产品详细信息
Positive Control: Hela cell lysate, HepG2 cell lysate, MG-63 cell lysate, Hela, HepG2, SW480, human liver tissue, human liver carcinoma tissue, human kidney tissue, mouse kidney tissue, mouse small intestine tissue.
Subcellular Location: Cell membrane
靶标信息
Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Alkaline phosphatase 2; alkaline phosphatase 2, liver; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; Phosphoamidase; Phosphocreatine phosphatase; tissue-nonspecific alkaline phosphatase; tissue-nonspecific ALP; Tissue-nonspecific ALP alkaline phosphatase; TNSALP
基因别名: Akp-2; Akp2; ALPL; AP-TNAP; APTNAP; HOPS; PHOA; TNAP; TNSALP
UniProt ID: (Human) P05186, (Rat) P08289, (Mouse) P09242
Entrez Gene ID: (Human) 249, (Rat) 25586, (Mouse) 11647
skeletal system development
osteoblast differentiation
endochondral ossification
developmental process involved in reproduction
dephosphorylation
response to lipopolysaccharide
response to vitamin D
response to antibiotic
response to glucocorticoid
cellular response to organic cyclic compound
cementum mineralization
metabolic process
