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AMPD3 Antibody (PA5-103597) in ICC/IF
Immunocytochemistry analysis of AMPD3 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with AMPD3 polyclonal antibody (Product # PA5-103597) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103597
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human AMPD3(Accession Q01432), corresponding to amino acid residues H514-A564.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852933
Product Specific Information
Antibody detects endogenous levels of total AMPD3.
Target Information
AMPD3 is a member of the AMP deaminase gene family. This protein is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. The protein is the erythrocyte (E) isoforms, whereas other family members isoforms predominate in muscle (M) and liver (L) cells. Mutations in this gene lead to the clinically asymptomatic, autosomal recessive condition erythrocyte AMP deaminase deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenosine monophosphate deaminase (isoform E); AMP aminohydrolase; AMP deaminase 3; AMP deaminase H-type; AMP deaminase isoform E; Ampd; Erythrocyte AMP deaminase; erythrocyte type AMP deaminase; erythrocyte-specific AMP deaminase; Heart-type AMPD; myoadenylate deaminase
Gene Aliases: AMPD3
UniProt ID: (Human) Q01432, (Rat) O09178, (Mouse) O08739
Entrez Gene ID: (Human) 272, (Rat) 25095, (Mouse) 11717
AMP catabolic process
IMP salvage
erythrocyte homeostasis
purine-containing compound salvage
ADP metabolic process
ATP metabolic process
GTP metabolic process
energy homeostasis
IMP biosynthetic process
AMP metabolic process
nucleotide metabolic process
purine ribonucleoside monophosphate biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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