Invitrogen

AMPK alpha-1 Monoclonal Antibody (2B7)

AMPK alpha-1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

AMPK alpha-1 Antibody (MA5-15815) in ICC/IF

Immunofluorescence analysis of AMPK alpha-1 was performed using 70% confluent log phase LNCap cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with AMPK alpha-1 Monoclonal Antibody (2B7)(Product # MA5-15815) at 1:100 dilution in 0.1% BSA and incubated overn... View More

AMPK alpha-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

AMPK alpha-1 Antibody in Western Blot (WB)

AMPK alpha-1 Antibody in Flow Cytometry (Flow)

Product Image Gallery

产品信息

MA5-15815

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:200-1:1,000

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2B7

抗原

Purified recombinant fragment of human PRKAA1 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11152993

产品详细信息

MA5-15815 targets PRKAA1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.

The MA5-15815 immunogen is purified recombinant fragment of human PRKAA1 expressed in E. Coli.

MA5-15815 detects PRKAA1 which has a predicted molecular weight of approximately 64kDa.

靶标信息

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Penta-O-Galloyl-β-D-Glucose in Pistacia integerrima Targets AMPK-ULK1 and ERK/STAT3 Signaling Axes to Induce ROS-Independent Autophagic Cell Death in Human Lung Cancer Cells.

Frontiers in pharmacology

Balkrishna A,Mulay VP,Verma S,Srivastava J,Lochab S,Varshney A

MA5-15815 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to propose PGG as a potential AMPK activator and STAT3 inhibitor that can be exploited further in developing adjuvant chemotherapeutics against lung cancer.

Sat Aug 06 00:00:00 UTC 2022

ACSL4 contributes to sevoflurane-induced ferroptotic neuronal death in SH-SY5Y cells via the 5' AMP-activated protein kinase/mammalian target of rapamycin pathway.

Annals of translational medicine

Cheng L,Zhu X,Liu Y,Zhu K,Lin K,Li F

MA5-15815 was used in Western Blot to show that downregulation of ACSL4 restrained sev-induced ferroptotic cell death via AMPK/mTOR signaling, providing the basis for an approach to alleviate sev-induced postoperative cognitive dysfunction (POCD).

Wed Sep 01 00:00:00 UTC 2021

Antifungal itraconazole ameliorates experimental autoimmune encephalomyelitis through a novel mechanism of action.

Advances in clinical and experimental medicine : official organ Wroclaw Medical University

Huang H,Tian X,Peng X,Huang L,Mei L,Zhan Y,Chen S,Wu H,Wei G,Cai X

MA5-15815 was used in Western Blotting to reveal a new method for treating MS using currently approved antifungal drugs.

Fri May 01 00:00:00 UTC 2020

α1AMP-Activated Protein Kinase Protects against Lipopolysaccharide-Induced Endothelial Barrier Disruption via Junctional Reinforcement and Activation of the p38 MAPK/HSP27 Pathway.

International journal of molecular sciences

Angé M,Castanares-Zapatero D,De Poortere J,Dufeys C,Courtoy GE,Bouzin C,Quarck R,Bertrand L,Beauloye C,Horman S

Published figure using AMPK alpha-1 monoclonal antibody (Product # MA5-15815) in Western Blot

Tue Aug 04 00:00:00 UTC 2020

Rotating magnetic field delays human umbilical vein endothelial cell aging and prolongs the lifespan of Caenorhabditis elegans.

Aging

Xu J,Liu K,Chen T,Zhan T,Ouyang Z,Wang Y,Liu W,Zhang X,Sun Y,Xu G,Wang X

MA5-15815 was used in Western Blotting to investigate the effects of a rotating magnetic field (RMF; 0.2 T, 4 Hz) on the growth of human umbilical vein endothelial cells (HUVECs) and Caenorhabditis elegans.

Fri Nov 22 00:00:00 UTC 2019

Phosphorylation of ULK1 by AMPK regulates translocation of ULK1 to mitochondria and mitophagy.

FEBS letters

Tian W,Li W,Chen Y,Yan Z,Huang X,Zhuang H,Zhong W,Chen Y,Wu W,Lin C,Chen H,Hou X,Zhang L,Sui S,Zhao B,Hu Z,Li L,Feng D

Published figure using AMPK alpha-1 monoclonal antibody (Product # MA5-15815) in Western Blot

Wed Jul 08 00:00:00 UTC 2015

生物信息学

蛋白别名: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; activated protein kinase; AMP -activate kinase alpha 1 subunit; AMP activated protein kinase; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha-1 chain; AMPK subunit alpha-1; AMPK subunit pAMPK; AMPK-alpha-2 chain; AMPK2; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; MGC33776; MGC57364; pAMPK; PRKAA; PRKAA2; protein kinase, AMP-activated, alpha 1 catalytic subunit; Tau-protein kinase PRKAA1

查看更多收起

基因别名: AI194361; AI450832; AL024255; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; PRKAA1

查看更多收起

UniProt ID: (Human) Q13131, (Rat) P54645, (Mouse) Q5EG47

查看更多收起

Entrez Gene ID: (Human) 5562, (Rat) 65248, (Mouse) 105787

查看更多收起

功能