Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 2
AMPK alpha-2 Antibody (MA5-37501) in ICC/IF
Immunocytochemistry/Immunofluorescent analysis of AMPK alpha-2 in Hela cells stained with AMPK alpha-2 Monoclonal Antibody (Product # MA5-37501). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AMPK alpha-2 primary antibody (1:25, 1h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400... View More
产品信息
MA5-37501
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
206CT5.4.2
抗原
PRKAA2 recombinant protein
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2897433
靶标信息
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMPK alpha 2; AMPK subunit alpha-2; AMPK-alpha-2 chain; EC 2.7.11.1; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 2 catalytic subunit
基因别名: AMPK; AMPK2; AMPKa2; PRKAA; PRKAA2
UniProt ID: (Human) P54646
Entrez Gene ID: (Human) 5563
chromatin binding
protein kinase activity
protein serine/threonine kinase activity
AMP-activated protein kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
histone serine kinase activity
metal ion binding
[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity
[acetyl-CoA carboxylase] kinase activity
non-receptor serine/threonine protein kinase
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
fatty acid biosynthetic process
cholesterol biosynthetic process
carnitine shuttle
response to stress
cell cycle arrest
signal transduction
lipid biosynthetic process
positive regulation of autophagy
response to muscle activity
Wnt signaling pathway
macroautophagy
positive regulation of macroautophagy
regulation of macroautophagy
cellular response to nutrient levels
negative regulation of TOR signaling
histone-serine phosphorylation
intracellular signal transduction
cellular response to drug
cellular response to glucose starvation
regulation of fatty acid biosynthetic process
glucose homeostasis
regulation of circadian rhythm
negative regulation of apoptotic process
positive regulation of glycolytic process
rhythmic process
fatty acid homeostasis
cellular response to prostaglandin E stimulus
regulation of signal transduction by p53 class mediator
regulation of energy homeostasis
