Invitrogen

ANO6 Polyclonal Antibody

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ANO6 Antibody (PA5-69345) in WB

Western blot analysis of ANO6 on mouse liver tissue lysate. The sample was probed with a ANO6 polyclonal antibody (Product # PA5-69345) using a primary antibody dilution of 1.0 µg/mL.

Product Image Gallery

ANO6 Antibody — Knockdown of ANO6 inhibited the activation of ERK signaling. After T98G and U87MG cells transfection with ANO6-KD 1 or ANO6-KD2, (A and B) the protein expression level of ERK and phospho-ERK was detected by Western blot assay; (C) the protein expression levels of ANO6 and ERK were detected by Western blot assay in cytoplasm and cell nucleus of T98G cells; (D and E) Histograms were used to represent statistical results of the protein expression levels of ANO6 and ERK in cytoplasm and cell nucleus of T98G cells; (F) the protein expression levels of ANO6 and ERK were detected by Western blot assay in cytoplasm and cell nucleus of U87MG cells. (G and H) Histograms were used to represent statistical results of the protein expression levels of ANO6 and ERK in cytoplasm and cell nucleus of U87MG cells; β-actin was used as a load control for cytoplasm. LaminB was used as a load control for cell nucleus. Data are presented as the mean standard deviation. **p<0.05 versus ANO6-KD1 group and ANO6-KD2 group. Abbreviations: NC is negative control, Rel. is relative. {KD}

Product Details

PA5-69345

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

synthetic peptide corresponding to a region of mouse ANO6 (aa 480-529).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2691066

Product Specific Information

This target displays homology in the following species: Guinea Pig: 77%; Human: 100%; Mouse: 92%; Pig: 93%; Rat: 92%

Target Information

May act as a calcium-activated chloride channel.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bacterial Endotoxin Activates the Coagulation Cascade through Gasdermin D-Dependent Phosphatidylserine Exposure.

Immunity

Yang X,Cheng X,Tang Y,Qiu X,Wang Y,Kang H,Wu J,Wang Z,Liu Y,Chen F,Xiao X,Mackman N,Billiar TR,Han J,Lu B

PA5-69345 was used in Western blot to examine the mechanisms underlying the activation of coagulation by lipopolysaccharide (LPS), the major cell-wall component of Gram-negative bacteria.

Tue Dec 17 00:00:00 UTC 2019

ANO6 promotes cell proliferation and invasion in glioma through regulating the ERK signaling pathway.

OncoTargets and therapy

Xuan ZB,Wang YJ,Xie J

PA5-69345 was used in Western Blotting to reveal that ANO6 activated ERK signalling pathway through promoting the nuclear translocation of ERK to increase the proliferation and invasion of glioblastoma cells.

Mon Apr 11 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: Anoctamin-6; SCAN channel; Small-conductance calcium-activated nonselective cation channel; Transmembrane protein 16F

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Gene Aliases: 2900059G15Rik; AA407480; Ano6; AW554778; F730003B03Rik; Tmem16f

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UniProt ID: (Mouse) Q6P9J9

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Entrez Gene ID: (Mouse) 105722

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Function(s)

calcium activated cation channel activity intracellular calcium activated chloride channel activity voltage-gated ion channel activity voltage-gated chloride channel activity chloride channel activity phospholipid scramblase activity protein homodimerization activity

Process(es)

dendritic cell chemotaxis activation of blood coagulation via clotting cascade transport ion transport cation transport chloride transport lipid transport blood coagulation phospholipid transport phospholipid scrambling positive regulation of bone mineralization bleb assembly regulation of ion transmembrane transport positive regulation of ion transmembrane transport purinergic nucleotide receptor signaling pathway bone mineralization involved in bone maturation sodium ion transmembrane transport negative regulation of cell volume pore complex assembly calcium activated phospholipid scrambling calcium activated phosphatidylserine scrambling calcium activated phosphatidylcholine scrambling calcium activated galactosylceramide scrambling calcium ion transmembrane transport phosphatidylserine exposure on apoptotic cell surface positive regulation of monocyte chemotaxis phosphatidylserine exposure on blood platelet cation transmembrane transport positive regulation of potassium ion export chloride transmembrane transport regulation of anion transmembrane transport positive regulation of endothelial cell apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ANO6 Polyclonal Antibody

ANO6 Antibody (PA5-69345) in WB

Product Details

PA5-69345

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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