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AP2 alpha Antibody (MA5-55880) in IHC (P)
Immunohistochemistry analysis of AP2 alpha in paraffin-embedded placenta tissue. Sample was incubated with AP2 alpha monoclonal antibody (Product # MA5-55880) at a dilution of 5 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room tempe... View More
Product Details
MA5-55880
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
K92029_15F12
Immunogen
Recombinant human TFAP2A (immunogen range is 131-430).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
Sequence of this protein is as follows: EDLLHGPHAL SSGLGDLSIH SLPHAIEEVP HVEDPGINIP DQTVIKKGPV SLSKSNSNAV SAIPINKDNL FGGVVNPNEV FCSVPGRLSL LSSTSKYKVT VAEVQRRLSP PECLNASLLG GVLRRAKSKN GGRSLREKLD KIGLNLPAGR RKAANVTLLT SLVEGEAVHL ARDFGYVCET EFPAKAVAEF LNRQHSDPNE QVTRKNMLLA TKQICKEFTD LLAQDRSPLG NSRPNPILEP GIQSCLTHFN LISHGFGSPA VCAAVTALQN YLTEALKAMD KMYLSNNPNS HTDNNAKSSD
Target Information
TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. TFAP2A is the only AP-2 protein required for early morphogenesis of the lens vesicle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; AP-2 transcription factor; AP2-alpha; ERF-1; FLJ51761; OTTHUMP00000214240; OTTHUMP00000214243; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha
Gene Aliases: AP-2; AP-2alpha; AP2TF; BOFS; TFAP2; TFAP2A
UniProt ID: (Human) P05549
Entrez Gene ID: (Human) 7020
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
chromatin binding
transcription coactivator activity
protein binding
transcription factor binding
protein homodimerization activity
sequence-specific DNA binding
transcription regulatory region DNA binding
protein dimerization activity
general transcription factor
negative regulation of transcription from RNA polymerase II promoter
kidney development
optic vesicle morphogenesis
optic cup structural organization
transcription from RNA polymerase II promoter
sensory perception of sound
negative regulation of cell proliferation
response to water deprivation
positive regulation of gene expression
retina layer formation
negative regulation of transcription by competitive promoter binding
Schwann cell development
trigeminal nerve development
oculomotor nerve formation
positive regulation of bone mineralization
response to lipopolysaccharide
embryonic forelimb morphogenesis
inner ear morphogenesis
negative regulation of apoptotic process
positive regulation of neuron apoptotic process
regulation of cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic cranial skeleton morphogenesis
palate development
bone morphogenesis
eyelid development in camera-type eye
positive regulation of tooth mineralization
cellular response to iron ion
negative regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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