Invitrogen

APH1 Polyclonal Antibody

APH1 Antibody in Immunocytochemistry (ICC/IF)

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APH1 Antibody (PA1-2010) in ICC/IF

Immunofluorescent analysis of APH-1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with APH-1 Rabbit Polyclonal Antibody (Product # PA1-2010) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More

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产品信息

PA1-2010

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

2 µg/mL

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse

已发表种属

Hamster, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide, KLH conjugated, corresponding to residues C (245) R R Q E D S R V M V Y S A L R I P P E D (265) of human APH-1aL/CT.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.69 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.005% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2227105

产品详细信息

PA1-2010 detects APH-1 in human and mouse samples.

PA1-2010 has been successfully used in immunofluorescent, immunoprecipitation, and Western blot procedures. By Western blot, this antibody detects a ~28 kDa protein representing APH-1 in mouse brain and kidney extracts.

The PA1-2010 immunogen is a Synthetic peptide, KLH conjugated, corresponding to residues C (245) R R Q E D S R V M V Y S A L R I P P E D (265) of the C-Terminal domain of the long isoform of APH-1a. This polyclonal antibody has been referred to as, O2C2 in the reference listed above.

靶标信息

APH1 is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). The gamma-secretase complex is minimally composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, although other components may exist. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Gossypetin ameliorates 5xFAD spatial learning and memory through enhanced phagocytosis against Aβ.

Alzheimer's research & therapy

Jo KW,Lee D,Cha DG,Oh E,Choi YH,Kim S,Park ES,Kim JK,Kim KT

PA1-2010 was used in Western Blotting to study how gossypetin showed protective effects against AD by enhancing microglial Aβ phagocytosis.

Fri Oct 21 00:00:00 UTC 2022

Physical and functional interaction between the α- and γ-secretases: A new model of regulated intramembrane proteolysis.

The Journal of cell biology

Chen AC,Kim S,Shepardson N,Patel S,Hong S,Selkoe DJ

PA1-2010 was used in western blot to identify and characterize a multiprotease complex containing both alpha- and gamma-secretase

Mon Dec 21 00:00:00 UTC 2015

Presenilin transmembrane domain 8 conserved AXXXAXXXG motifs are required for the activity of the γ-secretase complex.

The Journal of biological chemistry

Marinangeli C,Tasiaux B,Opsomer R,Hage S,Sodero AO,Dewachter I,Octave JN,Smith SO,Constantinescu SN,Kienlen-Campard P

PA1-2010 was used in western blot to investigate presenilin assembly and catalytic activity

Fri Mar 13 00:00:00 UTC 2015

Partial loss of presenilin impairs age-dependent neuronal survival in the cerebral cortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Watanabe H,Iqbal M,Zheng J,Wines-Samuelson M,Shen J

Published figure using APH1 polyclonal antibody (Product # PA1-2010) in Western Blot

Wed Nov 26 00:00:00 UTC 2014

Familial frontotemporal dementia-associated presenilin-1 c.548G>T mutation causes decreased mRNA expression and reduced presenilin function in knock-in mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Watanabe H,Xia D,Kanekiyo T,Kelleher RJ,Shen J

PA1-2010 was used in western blot to study the effects of a familial frontotemporal dementia-associated presenilin-1 c.548G>T mutation in knock-in mice

Wed Apr 11 00:00:00 UTC 2012

Inhibitors of γ-secretase stabilize the complex and differentially affect processing of amyloid precursor protein and other substrates.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Barthet G,Shioi J,Shao Z,Ren Y,Georgakopoulos A,Robakis NK

PA1-2010 was used in western blot to study the effect of gamma-secretase inhibitors on amyloid precursor protein

Thu Sep 01 00:00:00 UTC 2011

Calsenilin regulates presenilin 1/γ-secretase-mediated N-cadherin ε-cleavage and β-catenin signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Jang C,Choi JK,Na YJ,Jang B,Wasco W,Buxbaum JD,Kim YS,Choi EK

PA1-2010 was used in western blot to identify the role of Calsenilin in N-cadherin cleavage in neuroblastomas

Thu Dec 01 00:00:00 UTC 2011

Caspases-2 and -8 are involved in the presenilin1/gamma-secretase-dependent cleavage of amyloid precursor protein after the induction of apoptosis.

Journal of neuroscience research

Chae SS,Yoo CB,Jo C,Yun SM,Jo SA,Koh YH

PA1-2010 was used in western blot to investigate the involvement of caspase 2 and 8 in the presenilin/gamma-secretase activation and Abeta production in apoptosis

Thu Jul 01 00:00:00 UTC 2010

PI3K inhibition causes the accumulation of ubiquitinated presenilin 1 without affecting the proteasome activity.

Biochemical and biophysical research communications

Aoyagi N,Uemura K,Kuzuya A,Kihara T,Kawamata J,Shimohama S,Kinoshita A,Takahashi R

PA1-2010 was used in western blot to study the role of PI3K in ubiquitination and proteasome activity

Fri Jan 08 00:00:00 UTC 2010

Presenilins are enriched in endoplasmic reticulum membranes associated with mitochondria.

The American journal of pathology

Area-Gomez E,de Groof AJ,Boldogh I,Bird TD,Gibson GE,Koehler CM,Yu WH,Duff KE,Yaffe MP,Pon LA,Schon EA

PA1-2010 was used in western blot to study the expression and localization of presenilins in endoplasmic reticulum membranes

Sun Nov 01 00:00:00 UTC 2009

APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes.

The Journal of biological chemistry

Gu Y,Chen F,Sanjo N,Kawarai T,Hasegawa H,Duthie M,Li W,Ruan X,Luthra A,Mount HT,Tandon A,Fraser PE,St George-Hyslop P

PA1-2010 was used in western blot to investigate the role of APH-1 during the formation of presenilin and nicastrin complexes.

Fri Feb 28 00:00:00 UTC 2003

生物信息学

蛋白别名: anterior pharynx defective 1 homolog A; aph-1 homolog A, gamma secretase subunit; APH-1a; Aph-1alpha; APH1A gamma secretase subunit; Gamma-secretase subunit APH-1A; Presenilin-stabilization factor; RP4-790G17.3

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基因别名: 6530402N02Rik; APH-1; APH-1A; APH1A; CGI-78; PSF; UNQ579/PRO1141

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UniProt ID: (Human) Q96BI3, (Mouse) Q8BVF7

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Entrez Gene ID: (Human) 51107, (Mouse) 226548

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功能

endopeptidase activity protein binding peptidase activity

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