Invitrogen

APOE Polyclonal Antibody

APOE Antibody in Immunocytochemistry (ICC/IF)

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APOE Antibody (PA5-27088) in ICC/IF

Immunofluorescence analysis of APOE was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with APOE Rabbit Polyclonal Antibody (Product # PA5-27088) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilut... View More

APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

APOE Antibody in Immunoprecipitation (IP)

APOE Antibody in Immunohistochemistry (Frozen) (IHC (F))

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APOE Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lysates tested owing to their inherent genetic constitution. Relative expression of APOE was observed in Hep G2 and Caco-2 as compared to A-431 and Daudi using Anti-APOE Monoclonal Antibody (E6D7) (Product # PA5-27088) in Western Blot. {RE}

产品信息

PA5-27088

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:20,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

1:1,000-1:10,000

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a region within amino acids 255 and 317 of Human Apo E

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.66 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2544564

产品详细信息

Recommended positive controls: HepG2, Conditional medium from differentiated human primary preadipocyte, human plasma.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The P522R protective variant of PLCG2 promotes the expression of antigen presentation genes by human microglia in an Alzheimer's disease mouse model.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Claes C,England WE,Danhash EP,Kiani Shabestari S,Jairaman A,Chadarevian JP,Hasselmann J,Tsai AP,Coburn MA,Sanchez J,Lim TE,Hidalgo JLS,Tu C,Cahalan MD,Lamb BT,Landreth GE,Spitale RC,Blurton-Jones M,Davtyan H

PA5-27088 was used in Immunohistochemistry (Frozen) to provide the first evidence that the PLCG2-P522R variant increases the capacity of microglia to recruit T cells and present antigens, promoting a microglial transcriptional state that has recently been shown to be reduced in AD patient brains.

Sat Oct 01 00:00:00 UTC 2022

Plaque-associated human microglia accumulate lipid droplets in a chimeric model of Alzheimer's disease.

Molecular neurodegeneration

Claes C,Danhash EP,Hasselmann J,Chadarevian JP,Shabestari SK,England WE,Lim TE,Hidalgo JLS,Spitale RC,Davtyan H,Blurton-Jones M

PA5-27088 was used in Immunohistochemistry (Frozen) to indicate lipid droplet accumulation occurs in human DAM xMGs in AD, but is reduced in TREM2-R47H DAM xMGs, as it occurs secondary to TREM2-mediated changes in plaque proximity and reactivity.

Fri Jul 23 00:00:00 UTC 2021

Plaque-associated human microglia accumulate lipid droplets in a chimeric model of Alzheimer's disease.

Molecular neurodegeneration

Claes C,Danhash EP,Hasselmann J,Chadarevian JP,Shabestari SK,England WE,Lim TE,Hidalgo JLS,Spitale RC,Davtyan H,Blurton-Jones M

Fri Jul 23 00:00:00 UTC 2021

Morphological analysis of Apolipoprotein E binding to Aβ Amyloid using a combination of Surface Plasmon Resonance, Immunogold Labeling and Scanning Electron Microscopy.

BMC biotechnology

Islam T,Gharibyan AL,Lee CC,Olofsson A

Published figure using APOE polyclonal antibody (Product # PA5-27088) in Immunocytochemistry

Wed Dec 11 00:00:00 UTC 2019

Complement-Mediated Regulation of Apolipoprotein E in Cultured Human RPE Cells.

Investigative ophthalmology & visual science

Yang P,Skiba NP,Tewkesbury GM,Treboschi VM,Baciu P,Jaffe GJ

PA5-27088 was used in Immunocytochemistry to study the effect of complement activation on induction of apolipoprotein E accumulation in human retinal pigment epithelial cells.