Invitrogen

ARF1 Polyclonal Antibody

ARF1 Antibody in Immunocytochemistry (ICC/IF)

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ARF1 Antibody (PA1-127) in ICC/IF

Immunofluorescence analysis of ARF1 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ARF1 Rabbit Polyclonal Antibody(Product # PA1-127) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A270... View More

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARF1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

ARF1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with ARF1 siRNA and reduction of signal was observed in Western Blot using ARF1 Polyclonal Antibody (Product # PA1-127). {KD}

产品信息

PA1-127

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:3000

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫细胞化学 (ICC/IF)

5 µg/ml

免疫沉淀 (IP)

2 µg

产品规格

种属反应

Dog, Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide DWLSNQLRNQK

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2539892

产品详细信息

PA1-127 has been successfully used in Western blot in human, mouse, and canine samples, and in immunofluorescence and immunoprecipitation applications in human samples.

靶标信息

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Anti‑tumor properties of FoxO1 in YD‑9 oral squamous cell carcinoma cells.

Oncology reports

Kim YG,Seong C,Cho KA,Lee SM,Kim TJ,Kim HJ,Cho JH,Jung W,Jang S,Shin JC,Lee KH,Byun JS,Kim DY

PA1-127 was used in Western Blotting to demonstrate that FoxO1 exerted an anti‚tumor effect by suppressing proliferation and migration/invasion but promoting oxidative stress‚linked cell death in YD‚9 OSCC cells.

Thu Jun 01 00:00:00 UTC 2023

Analysis of Golgi Secretory Functions in Cancer.

Methods in molecular biology (Clifton, N.J.)

Banerjee P,Tan X,Russell WK,Kurie JM

PA1-127 was used in Western Blot to analyze Golgi secretory functions in genetically heterogeneous cancer cell lines.

Thu Dec 15 00:00:00 UTC 2022

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Science advances

Tan X,Banerjee P,Shi L,Xiao GY,Rodriguez BL,Grzeskowiak CL,Liu X,Yu J,Gibbons DL,Russell WK,Creighton CJ,Kurie JM

PA1-127 was used in Immunocytochemistry to show that p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Tue Jun 01 00:00:00 UTC 2021

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Science advances

Tan X,Banerjee P,Shi L,Xiao GY,Rodriguez BL,Grzeskowiak CL,Liu X,Yu J,Gibbons DL,Russell WK,Creighton CJ,Kurie JM

PA1-127 was used in Immunocytochemistry to show that p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Tue Jun 01 00:00:00 UTC 2021

Targeting N-myristoylation for therapy of B-cell lymphomas.

Nature communications

Beauchamp E,Yap MC,Iyer A,Perinpanayagam MA,Gamma JM,Vincent KM,Lakshmanan M,Raju A,Tergaonkar V,Tan SY,Lim ST,Dong WF,Postovit LM,Read KD,Gray DW,Wyatt PG,Mackey JR,Berthiaume LG

Published figure using ARF1 polyclonal antibody (Product # PA1-127) in Western Blot

Thu Oct 22 00:00:00 UTC 2020

BIG1 controls macrophage pro-inflammatory responses through ARF3-mediated PI(4,5)P2 synthesis.

Cell death & disease

Liu L,Zhang S,Wang Y,Bao W,Zhou Y,Dang W,Wang X,Li H,Cao X,You Y,Fang H,Shen X

PA1-127 was used in Western Blotting to reveal a crucial new role of BIG1 in regulating macrophage inflammation responses, and provide evidence for BIG1 as a potential promising therapeutic target in sepsis.

Fri May 15 00:00:00 UTC 2020

Cocaine-induced endocannabinoid signaling mediated by sigma-1 receptors and extracellular vesicle secretion.

eLife

Nakamura Y,Dryanovski DI,Kimura Y,Jackson SN,Woods AS,Yasui Y,Tsai SY,Patel S,Covey DP,Su TP,Lupica CR

PA1-127 was used in Western Blot to implicate the Sig-1R-ARF6 complex in control of EV release and demonstrate that cocaine-mediated 2-AG release can occur via EVs.