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ARHGEF1 Antibody (PA5-78821) in ICC/IF
Immunocytochemistry analysis of ARHGEF1 using anti-ARHGEF1 antibody (Product # PA5-78821). ARHGEF1 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-ARHGEF1 antibody (Product # PA5-78821) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Vi... View More
产品信息
PA5-78821
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the N-terminus of human ARHGEF1 (41-71aa EQNSQFQSLEQVKRRPAHLMALLQHVALQFE).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2745937
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
The Ras superfamily of GTPases, which can be subdivided into the Ras, Rho/Rac, Sar, Rab, ARF and Ran subfamilies, controls multiple aspects of cell function, including cytoskeletal rearrangement, nuclear signaling and cell growth. The Ras superfamily of GTPases function as regulated switches that toggle between a biologically active GTP-bound and an inactive GDP-bound form. This activation is catalyzed by guanine nucleotide exchange factors (GEFs). The Dbl-related proteins are a large family of structurally related molecules that have a common ability to catalyze GEF activity for specific members of the Ras family. Dbl-related proteins include FGD1, Lsc, RhoGEF p115, Lfc, Lbc and Brx. Lsc, Lbc and Lfc share sequence homology and show exchange activity toward Rho family GTPases. RhoGEF p115 catalyzes GEF activity for Rho but not Rac, Cdc42 or Ras GTPases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 115 kDa guanine nucleotide exchange factor; 115-kD protein; Lbc's second cousin; Lsc homolog; lymphoid blast crisis like 2; Lymphoid blast crisis-like 2; p115-RhoGEF; p115RhoGEF; Rho guanine nucleotide exchange factor (GEF) 1; Rho guanine nucleotide exchange factor 1; Sub1.5
基因别名: ARHGEF1; GEF1; LBCL2; LSC; P115-RHOGEF; SUB1.5
UniProt ID: (Human) Q92888, (Mouse) Q61210
Entrez Gene ID: (Human) 9138, (Mouse) 16801, (Rat) 60323
