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ASAP1 Antibody (600-401-GY1) in WB
Western Blot of Rabbit anti-ASAP1 antibody. Lane 1: ASAP1 transfected U118 lysate. Load: 35 µg per lane. Primary antibody: ASAP1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Block: BlockOut (p/n MB-073) overnight at 4°C. Predicted/Observed size: 125 kDa for ASAP1.
产品信息
600-401-GY1
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-ASAP1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-terminus region of human ASAP1 protein.
偶联物
形式
Liquid
浓度
1.04 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human ASAP1 protein. A BLAST analysis was used to suggest reactivity to human 100%, bovine 93%, and rat and mouse 87% homology for the immunogen sequence. Cross-reactivity from other sources has not been determined.
靶标信息
ASAP1, also known as Development and differentiation enhancing factor 1, is a protein with two ANK repeats, an Arf-GAP domain, a PH domain and a SH3 domain. ASAP1 is a homodimer known to interact with SRC and CRK and its activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2). It possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. It may coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2 and may also function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. It is widely expressed in most of the tissues. ASAP1 is a protooncogene involved in invasive breast cancer and in other types of invasive and malignant tumors, such as uveal melanomas.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein; 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; ADP-ribosylation factor-directed GTPase-activating protein 1; ARF GTPase-activating protein 1; Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; centaurin, beta 4; DEF-1; Development and differentiation-enhancing factor 1; PIP2-dependent ARF1 GAP
基因别名: AMAP1; ASAP1; CENTB4; DDEF1; KIAA1249; PAG2; PAP; ZG14P
UniProt ID: (Human) Q9ULH1
Entrez Gene ID: (Human) 50807
