Invitrogen

ASCL2 Polyclonal Antibody

ASCL2 Antibody in Immunocytochemistry (ICC/IF)

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ASCL2 Antibody (PA5-41489) in ICC/IF

Immunofluorescence analysis of ASCL2 was performed using HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ASCL2 Polyclonal Antibody (Product # PA5-41489) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 m... View More

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ASCL2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti- ASCL2 Polyclonal antibody (Product # PA5-41489) shows increased expression of ASCL2 in HT-29 cell line when compared to SH-SY5Y. {RE}

产品信息

PA5-41489

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the C-terminal of human ASCL2 (aa 121-170).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2605820

产品详细信息

Peptide sequence: RPQAVRPSAP RGPPGTTPVA ASPSRASSSP GRGGSSEPGS PRSAYSSDDS

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 93%; Human: 100%; Mouse: 79%; Pig: 100%; Rat: 93%

靶标信息

This gene is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. Involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: achaete-scute complex homolog 2; achaete-scute complex-like 2; Achaete-scute homolog 2; ASH-2; bHLHa45; Class A basic helix-loop-helix protein 45; H M bHLHa45; mammalian achaete/scute homologue 2; Mash2

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基因别名: ASCL2; ASH2; BHLHA45; HASH2; MASH2

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UniProt ID: (Human) Q99929

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Entrez Gene ID: (Human) 430

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein dimerization activity E-box binding basic helix-loop-helix transcription factor

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