Invitrogen

ASL Polyclonal Antibody

ASL Antibody in Immunocytochemistry (ICC/IF)

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ASL Antibody (PA5-22300) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of ASL was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ASL Polyclonal Antibody (Product # PA5-22300) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

ASL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-22300

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 13 and 261 (P04424) of ASL

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.78 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11152934

产品详细信息

Recommended positive controls: HeLa, HepG2, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12.

Predicted reactivity: Mouse (93%), Rat (88%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ASL encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Loss of the mitochondrial protein Abcb10 results in altered arginine metabolism in MEL and K562 cells and nutrient stress signaling through ATF4.

The Journal of biological chemistry

Miljkovic M,Seguin A,Jia X,Cox JE,Catrow JL,Bergonia H,Phillips JD,Stephens WZ,Ward DM

Published figure using ASL polyclonal antibody (Product # PA5-22300) in Western Blot

Sat Jul 01 00:00:00 UTC 2023

Characterization of the humanized FRG mouse model and development of an AAV-LK03 variant with improved liver lobular biodistribution.

Molecular therapy. Methods & clinical development

Cabanes-Creus M,Navarro RG,Liao SHY,Scott S,Carlessi R,Roca-Pinilla R,Knight M,Baltazar G,Zhu E,Jones M,Denisenko E,Forrest ARR,Alexander IE,Tirnitz-Parker JEE,Lisowski L

PA5-22300 was used in Immunohistochemistry (Frozen), Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that fine-tuning the HSPG attachment profile of AAV vectors can be a powerful tool for engineering their liver lobular transduction profiles.

Thu Mar 09 00:00:00 UTC 2023

GCN2 inhibition sensitizes arginine-deprived hepatocellular carcinoma cells to senolytic treatment.

Cell metabolism

Missiaen R,Anderson NM,Kim LC,Nance B,Burrows M,Skuli N,Carens M,Riscal R,Steensels A,Li F,Simon MC

PA5-22300 was used in Immunohistochemistry to suggest novel strategies to treat human liver cancers through targeting SLC7A1 and/or a combination of arginine restriction, inhibition of GCN2, and senolytic agents.

Tue Aug 02 00:00:00 UTC 2022

GCN2 inhibition sensitizes arginine-deprived hepatocellular carcinoma cells to senolytic treatment.

Cell metabolism

Missiaen R,Anderson NM,Kim LC,Nance B,Burrows M,Skuli N,Carens M,Riscal R,Steensels A,Li F,Simon MC

Tue Aug 02 00:00:00 UTC 2022

l-Citrulline Metabolism in Mice Augments CD4⁺ T Cell Proliferation and Cytokine Production In Vitro, and Accumulation in the Mycobacteria-Infected Lung.

Frontiers in immunology

Lange SM,McKell MC,Schmidt SM,Hossfeld AP,Chaturvedi V,Kinder JM,McAlees JW,Lewkowich IP,Way SS,Turner J,Qualls JE

PA5-22300 was used in Western Blotting to establish a contribution for l-arginine synthesis by T cells during mycobacterial infection, and implicate l-citrulline as a potential immuno-nutrient to modulate host immunity.

Wed Nov 20 00:00:00 UTC 2019

Differential Requirements for L-Citrulline and L-Arginine during Antimycobacterial Macrophage Activity.

Journal of immunology (Baltimore, Md. : 1950)

Rapovy SM,Zhao J,Bricker RL,Schmidt SM,Setchell KD,Qualls JE

PA5-22300 was used in Western Blotting to define the metabolic signature of mycobacteria-infected murine macrophages supplied L-arginine, L-citrulline, or both amino acids.