Invitrogen

ASM Polyclonal Antibody

ASM Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

ASM Antibody (PA5-95730) in ICC/IF

Immunofluorescence analysis of ASM in NIH-3T3 cells. Samples were incubated with ASM Polyclonal antibody (Product # PA5-95730) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Product Image Gallery

产品信息

PA5-95730

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:100

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1 / ASM (NP_0005343)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.05 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2807532

产品详细信息

Positive Samples: THP-1, HepG2, U-251MG, MCF-7, Mouse brain, Mouse liver, Rat brain, Rat liver; Cellular Location: Lysosome

Immunogen sequence: PARLHRIVPR LRDVFGWGNL TCPICKGLFT AINLGLKKEP NVARVGSVAI KLCNLLKIAP PAVCQSIVHL FEDDMVEVWR RSVLSPSEAC GLLLGSTCGH WDIFSSWNIS LPTVPKPPPK PPSPPAPGAP VSRILFLTDL HWDHDYLEGT DPDCADPLCC RRGSGLPPAS RPGAGYWGEY SKCDLPLRTL ESLLSGLGPA GPFDMVYWTG DIPAHDVWHQ TRQDQLRALT TVTALVRKFL GPVPVYPAVG NHESTPVNSF PPPFIEGNHS S

靶标信息

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Three transcript variants encoding two different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

High levels of modified ceramides are a defining feature of murine and human cancer cachexia.

Journal of cachexia, sarcopenia and muscle

Morigny P,Zuber J,Haid M,Kaltenecker D,Riols F,Lima JDC,Simoes E,Otoch JP,Schmidt SF,Herzig S,Adamski J,Seelaender M,Berriel Diaz M,Rohm M

PA5-95730 was used in Western Blotting to identify specific lipid species as a hallmark of CCx by performing a broad range lipid analysis of plasma from well-established CCx mouse models as well as cachectic and weight stable cancer patients.