Invitrogen

ASPM Polyclonal Antibody

ASPM Antibody in Immunocytochemistry (ICC/IF)

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ASPM Antibody (PA5-99790) in ICC/IF

Immunocytochemistry analysis of ASPM in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ASPM polyclonal antibody (Product # PA5-99790) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

ASPM Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-99790

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

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免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

流式细胞分析 (Flow)

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产品规格

种属反应

Human, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human ASPM(Accession Q8IZT6), corresponding to amino acid residues L1261-R1311.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2818723

产品详细信息

Antibody detects endogenous levels of total ASPM.

靶标信息

This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Single-cell sequencing analysis reveals development and differentiation trajectory of Schwann cells manipulated by M. leprae.

PLoS neglected tropical diseases

Ma S,Mi Z,Wang Z,Sun L,Liu T,Shi P,Wang C,Xue X,Chen W,Wang Z,Yu Y,Zhang Y,Bao F,Wang N,Wang H,Xia Q,Liu H,Sun Y,Zhang F

PA5-99790 was used in Flow Cytometry to understand the critical biological processes underlying reprogramming and lipid metabolism during M. leprae infection of SCs, and increase understanding of the disease-causing mechanisms at play in leprosy patients as well as revealing potential new therapeutic strategies.

Sat Jul 01 00:00:00 UTC 2023

Single-cell sequencing analysis reveals development and differentiation trajectory of Schwann cells manipulated by M. leprae.

PLoS neglected tropical diseases

Ma S,Mi Z,Wang Z,Sun L,Liu T,Shi P,Wang C,Xue X,Chen W,Wang Z,Yu Y,Zhang Y,Bao F,Wang N,Wang H,Xia Q,Liu H,Sun Y,Zhang F

Sat Jul 01 00:00:00 UTC 2023

生物信息学

蛋白别名: Abnormal spindle protein homolog; Abnormal spindle-like microcephaly-associated protein; asp (abnormal spindle) homolog, microcephaly associated; asp (abnormal spindle)-like, microcephaly associated; Asp homolog; calmodulin binding protein 1

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基因别名: ASP; ASPM; Calmbp1; MCPH5

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UniProt ID: (Human) Q8IZT6

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Entrez Gene ID: (Human) 259266, (Rat) 289054

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功能

calmodulin binding molecular_function

参与通路