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ATF4 Antibody (PA5-120026) in WB
Western blot analysis of ATF4 in extracts of HeLa cells. Samples were incubated with ATF4 Polyclonal antibody (Product # PA5-120026) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. HeLa cells were treated by tunicamycin (2 µg/mL) for 8 hours. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.
产品信息
PA5-120026
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATF4 (NP_001666.2).
偶联物
形式
Liquid
浓度
0.44 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2913598
产品详细信息
Positive test controls include: HeLa. The target is usually found in the following locations: Cell membrane, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Immunogen sequence: MTEMSFLSSE VLVGDLMSPF DQSGLGAEES LGLLDDYLEV AKHFKPHGFS SDKAKAGSSE WLAVDGLVSP SNNSKEDAFS GTDWMLEKMD LKEFDLDALL GIDDLETMPD DLLTTLDDTC DLFAPLVQET NKQPPQTVNP IGHLPESLTK PDQVAPFTFL QPLPLSPGVL SSTPDHSFSL ELGSEVDITE GDRKPDYTAY VAMIPQCIKE EDTPSDNDSG ICMSPESYLG SPQHSPSTRG SPNRSLPSPG VLCGSARPKP YDPPGEKMVA AKVKGEKLDK KLKKMEQNKT AATRYRQKKR AEQEALTGEC KELEKKNEAL KERADSLAKE IQYLKDLIEE VRKARGKKRV P
靶标信息
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Activating transcription factor 4; ATF 4; ATF4 protein; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB 2; CREB-2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; OTTHUMP00000199130; tax-re; tax-responsive enhancer element B67; Tax-responsive enhancer element-binding protein 67; TaxREB67
基因别名: ATF4; CREB-2; CREB2; TAXREB67; TXREB
UniProt ID: (Human) P18848
Entrez Gene ID: (Human) 468
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein C-terminus binding
leucine zipper domain binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
DNA-binding transcription factor
gluconeogenesis
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular amino acid metabolic process
gamma-aminobutyric acid signaling pathway
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
negative regulation of translational initiation in response to stress
circadian regulation of gene expression
cellular response to amino acid starvation
cellular response to UV
response to endoplasmic reticulum stress
positive regulation of transcription from RNA polymerase II promoter in response to stress
positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress
PERK-mediated unfolded protein response
cellular response to glucose starvation
mRNA transcription from RNA polymerase II promoter
positive regulation of apoptotic process
negative regulation of potassium ion transport
positive regulation of neuron apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase I promoter
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
negative regulation of oxidative stress-induced neuron death
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
response to manganese-induced endoplasmic reticulum stress
