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ATG4D Antibody (PA5-98576) in ICC/IF
Immunofluorescent analysis of ATG4D in PC-3 cells using a ATG4D polyclonal antibody (Product # PA5-98576) at a dilution of 1:233. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
产品信息
PA5-98576
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human Cysteine protease ATG4D protein (14-43AA)
偶联物
形式
Liquid
浓度
3.73 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2813189
靶标信息
Atg4D (autophagy-related gene 4D), also known as APG4D or AUTL4, is a 474 amino acid protein that localizes to the cytoplasm and belongs to the C-54 family of cysteine proteases. Expressed predominately in skeletal muscle, but also present in testis, Atg4D functions as a cysteine protease that is required for autophagy and functions to specifically cleave the C-terminal region of target proteins, thereby allowing the target proteins to bind to autophagosomes. The enzymatic activity of Atg4D is inhibited by Nethylmaleimide, a thiol reactive compound that is capable of modifying cystine residues in proteins and peptides.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: APG4 (ATG4) autophagy-related homolog D; APG4 autophagy 4 homolog D; ATG4 autophagy related 4 homolog D; AUT-like 4 cysteine endopeptidase; AUT-like 4, cysteine endopeptidase; Autophagin-4; autophagy related 4D, cysteine peptidase; autophagy-related 4D; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D; Cysteine protease ATG4D; cysteine protease involved in autophagy; cysteine protease involved in autophagy APG4-D; HsAPG4D
基因别名: 9830134P12Rik; APG4-D; APG4D; ATG4D; Atg4dl; AUTL4
UniProt ID: (Human) Q86TL0, (Mouse) Q8BGV9
Entrez Gene ID: (Human) 84971, (Mouse) 235040
