Invitrogen

ATG7 Polyclonal Antibody

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ATG7 Antibody (PA5-17216) in WB

Western blot was performed using Anti-ATG7 Polyclonal Antibody (Product # PA5-17216) and a 70 kDa band corresponding to ATG7 was observed across cell lines tested and increased upon Chloroquine treatment. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with Chloroquine (50nM for 15 hr) (Lane 2), HCT 116 (Lane 3) and HCT 116 treated with Chloroquine (50nM for 15 hr) (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membr... View More

ATG7 Antibody in Immunoprecipitation (IP)

Product Image Gallery

ATG7 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using ATG7 Polyclonal Antibody (Product # PA5-17216), shows increased expression of proteins in HeLa and HCT 116 cell lines upon Chloroquine treatment. {TM}

产品信息

PA5-17216

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫沉淀 (IP)

3 µL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the amino terminus of Atg7

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

8 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 100µg/mL BSA, 0.15M NaCl, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10984892

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Knockdown of VDAC1 alleviates the cognitive dysfunction secondary to sepsis-associated encephalopathy.

American journal of translational research

Cai M,Du B,Si Y,Miao J,Ge J,Zhang J,Song J,Bao H

PA5-17216 was used in Western Blot to elucidate the underlying mechanisms where knockdown of voltage dependent anion channel (VDAC1) alleviates the cognitive dysfunction caused by sepsis-associated encephalopathy.

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Vasorin/ATIA Promotes Cigarette Smoke-Induced Transformation of Human Bronchial Epithelial Cells by Suppressing Autophagy-Mediated Apoptosis.

Translational oncology

Chen W,Wang Q,Xu X,Saxton B,Tessema M,Leng S,Choksi S,Belinsky SA,Liu ZG,Lin Y

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Synergistic anticancer effect of cisplatin and Chal-24 combination through IAP and c-FLIPL degradation, Ripoptosome formation and autophagy-mediated apoptosis.

Oncotarget

Shi S,Wang Q,Xu J,Jang JH,Padilla MT,Nyunoya T,Xing C,Zhang L,Lin Y

PA5-17216 was used in western blot to investigate if Chal-24 can be combined with cisplatin for better cancer therapy

Fri Jan 30 00:00:00 UTC 2015

A JNK-mediated autophagy pathway that triggers c-IAP degradation and necroptosis for anticancer chemotherapy.

Oncogene

He W,Wang Q,Srinivasan B,Xu J,Padilla MT,Li Z,Wang X,Liu Y,Gou X,Shen HM,Xing C,Lin Y

PA5-17216 was used in western blot to identify a novel anticancer mechanism that functions through autophagy-mediated necroptosis

Thu Jun 05 00:00:00 UTC 2014

Attenuation of TNFSF10/TRAIL-induced apoptosis by an autophagic survival pathway involving TRAF2- and RIPK1/RIP1-mediated MAPK8/JNK activation.

Autophagy

He W,Wang Q,Xu J,Xu X,Padilla MT,Ren G,Gou X,Lin Y

Published figure using ATG7 polyclonal antibody (Product # PA5-17216) in Western Blot

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生物信息学

蛋白别名: APG7-like; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog; autophagy related 7 homolog; autophagy-related 7; Autophagy-related protein 7; DKFZp434N0735; hAGP7; Ubiquitin-activating enzyme E1-like protein; Ubiquitin-like modifier-activating enzyme ATG7

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基因别名: 1810013K23Rik; Agp7; APG7-LIKE; APG7L; ATG7; Atg7l; Gm21553; GSA7

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UniProt ID: (Human) O95352, (Rat) Q641Y5, (Mouse) Q9D906

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Entrez Gene ID: (Human) 10533, (Rat) 312647, (Mouse) 74244

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功能

ubiquitin activating enzyme activity protein binding transcription factor binding Atg12 activating enzyme activity Atg8 activating enzyme activity protein homodimerization activity molecular_function small protein activating enzyme activity ubiquitin-protein ligase

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