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ATG9A Antibody (MA1-149-A555) in ICC/IF
Immunofluorescent analysis of ATG9A (red) in 0.1% serum starved (left) and non-serum-starved (right) U2OS cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were stained with a ATG9A mouse monoclonal antibody (Product # MA1-149-A555) at a concentration of 5 µg/mL in blocking buffer for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Therm... View More
产品信息
MA1-149-A555
产品规格
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
14F2 8B1
抗原
peptide sequence HPEPVPEEGSEDELPPQVHK of human ATG9A C-terminus
偶联物
激发/发射光谱
553/568 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Dry ice (international)
RRID
AB_2723939
产品详细信息
MA1-149A555 was produced in Armenian hamster and detects ATG9 in human, rat and mouse samples. In Immunofluorescence applications, MA1-149A555 shows accumulation and redestribution of ATG9 in response to starvation-induced autophagosome assembly.
靶标信息
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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