Invitrogen

ATGL Monoclonal Antibody (K.751.7)

ATGL Antibody in Immunocytochemistry (ICC/IF)

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ATGL Antibody (MA5-14990) in ICC/IF

Immunofluorescence analysis of ATGL was performed using 70% confluent log phase 3T3-L1 and 3T3-L1 differentiated to adipocytes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ATGL Monoclonal Antibody (K.751.7) (Product # MA5-14990) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Anti... View More

ATGL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATGL Antibody in Western Blot, Immunoprecipitation (WB, IP)

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ATGL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using ATGL Monoclonal Antibody (K.751.7) (Product # MA5-14990) shows increased expression of ATGL in 3T3-L1 differentiated adipocytes in comparison to 3T3-L1. {RE}

产品信息

MA5-14990

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:50

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

1:50

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产品规格

种属反应

Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

K.751.7

抗原

Synthetic peptide corresponding to residues surrounding Pro469 of mouse ATGL

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

23 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_10974333

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Activation of Cx43 Hemichannels Induces the Generation of Ca²⁺ Oscillations in White Adipocytes and Stimulates Lipolysis.

International journal of molecular sciences

Turovsky EA,Varlamova EG,Turovskaya MV

MA5-14990 was used in Western Blotting to investigate the mechanisms of Ca2+ oscillation generation upon activation of connexin-43 and regulation of the lipolysis/lipogenesis balance in white adipocytes through vesicular ATP release.

Wed Jul 28 00:00:00 UTC 2021

Activation of Cx43 Hemichannels Induces the Generation of Ca²⁺ Oscillations in White Adipocytes and Stimulates Lipolysis.

International journal of molecular sciences

Turovsky EA,Varlamova EG,Turovskaya MV

Wed Jul 28 00:00:00 UTC 2021

Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.

Scientific reports

An M,Park YH,Lim YH

MA5-14990 was used in Western Blotting to investigate the antiobesity and antidiabetic effects of the dairy bacterium P. freudenreichii MJ2 isolated from raw milk using 3T3-L1 cells and high-fat diet (HFD)-induced obese mice.

Thu Jan 28 00:00:00 UTC 2021

Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.

Scientific reports

An M,Park YH,Lim YH

Thu Jan 28 00:00:00 UTC 2021

Myocardial Adipose Triglyceride Lipase Overexpression Protects against Burn-Induced Cardiac Lipid Accumulation and Injury.

Oxidative medicine and cellular longevity

Li L,Zhang X,Zhang Q,Jia J,Zhang J,Zhang D,Song H,Chen B,Hu J,Huang Y

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Fri Dec 13 00:00:00 UTC 2019

Atgl deficiency induces podocyte apoptosis and leads to glomerular filtration barrier damage.

The FEBS journal

Chen W,Jiang Y,Han J,Hu J,He T,Yan T,Huang N,Zhang Q,Mei H,Liao Y,Huang Y,Chen B

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Sat Apr 01 00:00:00 UTC 2017

Fat-specific protein 27 (FSP27) interacts with adipose triglyceride lipase (ATGL) to regulate lipolysis and insulin sensitivity in human adipocytes.

The Journal of biological chemistry

Grahn THM,Kaur R,Yin J,Schweiger M,Sharma VM,Lee MJ,Ido Y,Smas CM,Zechner R,Lass A,Puri V

MA5-14990 was used in immunoprecipitation to study the interaction of FSP27 with adipose triglyceride lipase and its role in regulating adipocyte lipolysis and insulin signaling

Fri Apr 25 00:00:00 UTC 2014

Fat-specific protein 27 (FSP27) interacts with adipose triglyceride lipase (ATGL) to regulate lipolysis and insulin sensitivity in human adipocytes.

The Journal of biological chemistry

Grahn THM,Kaur R,Yin J,Schweiger M,Sharma VM,Lee MJ,Ido Y,Smas CM,Zechner R,Lass A,Puri V

MA5-14990 was used in immunoprecipitation to study the interaction of FSP27 with adipose triglyceride lipase and its role in regulating adipocyte lipolysis and insulin signaling

Fri Apr 25 00:00:00 UTC 2014

生物信息学

蛋白别名: Adipose triglyceride lipase; ATGL DESNUTRIN; Calcium-independent phospholipase A2-zeta; Desnutrin; DKFZp667M109; IPLA2 zeta; iPLA2-zeta; Patatin-like phospholipase domain-containing protein 2; PEDF R; plpl; plpl2; transport-secretion protein; triglyceride hydrolase; TTS 2.2

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基因别名: 0610039C21Rik; 1110001C14Rik; Atgl; Pnpla2; RGD1309044; TTS-2.2

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UniProt ID: (Rat) P0C548, (Mouse) Q8BJ56

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Entrez Gene ID: (Rat) 361676, (Mouse) 66853

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功能

triglyceride lipase activity protein binding hydrolase activity phospholipase

参与通路

negative regulation of sequestering of triglyceride positive regulation of triglyceride catabolic process triglyceride catabolic process lipid metabolic process metabolic process lipid catabolic process lipid storage lipid particle organization