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ATOH8 Antibody (PA5-99779) in ICC/IF
Immunocytochemistry analysis of ATOH8 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ATOH8 polyclonal antibody (Product # PA5-99779) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-99779
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ATOH8(Accession Q96SQ7), corresponding to amino acid residues Q264-G314.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2818712
产品详细信息
Antibody detects endogenous levels of total ATOH8.
靶标信息
Basic helix-loop-helix (bHLH) transcription factors play important roles in differentiation processes during embryonic development of vertebrates. ATOH8 (MATH6) is a tissue-restricted member of the atonal superfamily of bHLH transcription factors that exhibits 43-57% identity in the bHLH domain with other mammalian atonal paralogs including the NeuroD and Neurogenin factors. In the mouse, ATOH8 has been implicated in the specification and differentiation of neuronal cell lineages in the brain and may also participate in kidney development. Recent studies show that ATOH8 is a novel component of the pancreatic transcriptional network during embryonic development and suggest a potential role as a modulator of the differentiation program initiated by the pro-endocrine factor Neurog3. It is indispensable for early embryonic development, suggesting a more widespread function for this factor in tissue-specific differentiation processes that are dependent on class II bHLH genes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: atonal homolog 6; atonal homolog 8; atonal homolog bHLH transcription factor 8; basic helix loop helix transcription factor 6; basic helix-loop-helix transcription factor 6; bHLHa21; Class A basic helix-loop-helix protein 21; hATH6; Helix-loop-helix protein hATH-6; Protein atonal homolog 8; Transcription factor ATOH8
基因别名: ATH6; ATOH8; BHLHA21; HATH6
UniProt ID: (Human) Q96SQ7
Entrez Gene ID: (Human) 84913
formation of primary germ layer
negative regulation of endothelial cell proliferation
transcription, DNA-templated
nervous system development
positive regulation of endothelial cell migration
cell differentiation
tube formation
positive regulation of endothelial cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
myoblast proliferation
SMAD protein signal transduction
