Invitrogen

ATP Citrate Lyase Polyclonal Antibody

ATP Citrate Lyase Antibody in Immunocytochemistry (ICC/IF)

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ATP Citrate Lyase Antibody (PA5-29495) in ICC/IF

Immunofluorescence analysis of ATP Citrate Lyase was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29495) at 5 µg/mL in 0.1% BSA, incubated at 4 degree... View More

ATP Citrate Lyase Antibody in Western Blot (WB)

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产品信息

PA5-29495

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 743 and 1038 of Human ATP citrate lyase

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2546971

产品详细信息

Recommended positive controls: HeLa, Raji, mouse brain, PC-12, Rat-2.

Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (91%), Pig (98%), Chicken (95%), Sheep (99%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

BCAA Supplementation in Mice with Diet-induced Obesity Alters the Metabolome Without Impairing Glucose Homeostasis.

Endocrinology

Lee J,Vijayakumar A,White PJ,Xu Y,Ilkayeva O,Lynch CJ,Newgard CB,Kahn BB

PA5-29495 was used in Western Blotting to highlight major differences in the effects of BCAAs on glucose homeostasis in diet-induced obese mice versus data reported in obese rats and in humans.

Thu Jul 01 00:00:00 UTC 2021

Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation.

Molecular metabolism

Hughey CC,James FD,Wang Z,Goelzer M,Wasserman DH

PA5-29495 was used in Western Blotting to test that nutrient flux pivots from glucose production to pathways that incorporate and metabolise methyl groups in GNMT-null mice with hepatocellular carcinoma.

Wed May 01 00:00:00 UTC 2019

Glycine N-methyltransferase deletion in mice diverts carbon flux from gluconeogenesis to pathways that utilize excess methionine cycle intermediates.

The Journal of biological chemistry

Hughey CC,Trefts E,Bracy DP,James FD,Donahue EP,Wasserman DH

PA5-29495 was used in Western Blotting to demonstrate that glycine N-methyltransferase promotes nutrient channeling away from glucose formation toward pathways that utilize the elevated S-adenosylmethionine.

Fri Jul 27 00:00:00 UTC 2018

Glycine N-methyltransferase deletion in mice diverts carbon flux from gluconeogenesis to pathways that utilize excess methionine cycle intermediates.

The Journal of biological chemistry

Hughey CC,Trefts E,Bracy DP,James FD,Donahue EP,Wasserman DH

Fri Jul 27 00:00:00 UTC 2018

Extended Multiplexing of Tandem Mass Tags (TMT) Labeling Reveals Age and High Fat Diet Specific Proteome Changes in Mouse Epididymal Adipose Tissue.

Molecular & cellular proteomics : MCP

Plubell DL,Wilmarth PA,Zhao Y,Fenton AM,Minnier J,Reddy AP,Klimek J,Yang X,David LL,Pamir N

PA5-29495 was used in Western Blotting to identify that both age-related and high fat diet specific proteomic signatures highlight a pronounced involvement of arginine metabolism in response to advancing age, and branched chain amino acid metabolism in early response to high fat feeding.