Invitrogen

ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (4H6L2)

ATP Citrate Lyase Antibody in Immunocytochemistry (ICC/IF)

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ATP Citrate Lyase Antibody (702731) in ICC/IF

For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous ATP Citrate Lyase using Anti- ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (Product # 702731, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained ... View More

ATP Citrate Lyase Antibody in Western Blot (WB)

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产品信息

702731

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

查看2篇已发表文章 2篇已发表文章

染色质免疫沉淀实验 (ChIP)

查看6篇已发表文章 6篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

体外检测 (IV)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse, Pig, Plant

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

4H6L2

抗原

Protein corresponding to human ATP Citrate Lyase [aa1-aa425]

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2716887

产品详细信息

This antibody is predicted to react with Monkey, Sheep, Cat

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Glutamate triggers the expression of functional ionotropic and metabotropic glutamate receptors in mast cells.

Cellular & molecular immunology

Alim MA,Grujic M,Ackerman PW,Kristiansson P,Eliasson P,Peterson M,Pejler G

702731 was used in Microarray to provide in vivo evidence supporting a functional glutamate-glutamate receptor axis in the mast cells of injured tendons.

Fri Oct 01 00:00:00 UTC 2021

Transcription factor FOXP2 is a flow-induced regulator of collecting lymphatic vessels.

The EMBO journal

Hernández Vásquez MN,Ulvmar MH,González-Loyola A,Kritikos I,Sun Y,He L,Halin C,Petrova TV,Mäkinen T

702731 was used in Flow Cytometry to identify FOXP2 as a new flow-induced transcriptional regulator of collecting lymphatic vessel morphogenesis and highlight the existence of unique transcription factor codes in the establishment of vessel-type-specific endothelial cell identities.

Tue Jun 15 00:00:00 UTC 2021

Distinct B cell subsets in Peyer's patches convey probiotic effects by Limosilactobacillus reuteri.

Microbiome

Liu HY,Giraud A,Seignez C,Ahl D,Guo F,Sedin J,Walden T,Oh JH,van Pijkeren JP,Holm L,Roos S,Bertilsson S,Phillipson M

702731 was used in Functional Assay to investigate how peroral administration of probiotic bacterium Limosilactobacillus reuteri R2LC affects B lymphocytes and IgA induction in the PPs, as well as the downstream consequences on intestinal microbiota and susceptibility to inflammation.

Sun Oct 03 00:00:00 UTC 2021

Identification of human glucocorticoid response markers using integrated multi-omic analysis from a randomized crossover trial.

eLife

Chantzichristos D,Svensson PA,Garner T,Glad CA,Walker BR,Bergthorsdottir R,Ragnarsson O,Trimpou P,Stimson RH,Borresen SW,Feldt-Rasmussen U,Jansson PA,Skrtic S,Stevens A,Johannsson G

702731 was used in Immunocytochemistry, Western Blot, ChIP Assay to identify and validate circulating biomarkers of glucocorticoid action.

Tue Apr 06 00:00:00 UTC 2021

Transcriptome profiling data reveals ubiquitin-specific peptidase 9 knockdown effects.

Data in brief

Glaab E,Antony P,Köglsberger S,Forster JI,Cordero-Maldonado ML,Crawford A,Garcia P,Buttini M

702731 was used in Flow cytometry/Cell sorting to conduct microarray experiments on USP9 to report on its molecular mechanisms.

Thu Aug 01 00:00:00 UTC 2019

Targeting Zfp148 activates p53 and reduces tumor initiation in the gut.

Oncotarget

Nilton A,Sayin VI,Zou ZV,Sayin SI,Bondjers C,Gul N,Agren P,Fogelstrand P,Nilsson O,Bergo MO,Lindahl P

702731 was used in ChIP assay to suggest that Zfp148 controls the fate of newly transformed intestinal tumor cells by repressing p53 and that targeting Zfp148 might be useful in the treatment of colorectal cancer.

Tue Aug 30 00:00:00 UTC 2016

Gene Expression in Placentas From Nondiabetic Women Giving Birth to Large for Gestational Age Infants.

Reproductive sciences (Thousand Oaks, Calif.)

Ahlsson F,Åkerud H,Schijven D,Olivier J,Sundström-Poromaa I

702731 was used in ChIP Assay, Immunocytochemistry, Western Blot to compare placental gene expression between healthy, nondiabetic mothers (n = 22) giving birth to LGA infants and body mass index-matched mothers (n = 24) giving birth to appropriate for gestational age infants.

Thu Oct 01 00:00:00 UTC 2015

INF-γ Enhances Nox2 Activity by Upregulating phox Proteins When Applied to Differentiating PLB-985 Cells but Does Not Induce Nox2 Activity by Itself.

PloS one

Ellison MA,Thurman G,Gearheart CM,Seewald RH,Porter CC,Ambruso DR

702731 was used in ChIP assay to indicate that interferon-γ has complex effects on phox protein expression and that these are different in cells undergoing terminal differentiation.

Thu May 12 00:00:00 UTC 2016

A modified Glenn shunt reduces venous congestion during acute right ventricular failure due to pulmonary banding: a randomized experimental study.

Interactive cardiovascular and thoracic surgery

Vikholm P,Schiller P,Hellgren L

702731 was used in ChIP assay to study the alterations in genetic expression in the right ventricle due to pressure and volume overload.

Tue Apr 01 00:00:00 UTC 2014

A modified Glenn shunt improves haemodynamics in acute right ventricular failure in an experimental model.

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery

Vikholm P,Schiller P,Johansson J,Hellgren L

702731 was used in ChIP Assay, Immunocytochemistry, Western Blot to investigate the haemodynamic effect of a modified Glenn shunt on induced right ventricular (RV) failure.

Fri Mar 01 00:00:00 UTC 2013

Role of camalexin, indole glucosinolates, and side chain modification of glucosinolate-derived isothiocyanates in defense of Arabidopsis against Sclerotinia sclerotiorum.

The Plant journal : for cell and molecular biology

Stotz HU,Sawada Y,Shimada Y,Hirai MY,Sasaki E,Krischke M,Brown PD,Saito K,Kamiya Y

702731 was used in Immunocytochemistry, Western Blot, ChIP Assay to show that, with respect to aliphatic glucosinolate-derived isothiocyanates, toxicity against the pathogenic ascomycete Sclerotinia sclerotiorum depends on side chain structure.

Fri Jul 01 00:00:00 UTC 2011

生物信息学

蛋白别名: ACL; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; Citrate cleavage enzyme

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基因别名: ACL; ACLY; ATPCL; CLATP

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UniProt ID: (Human) P53396

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Entrez Gene ID: (Human) 47

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功能

ATP citrate synthase activity protein binding ATP binding metal ion binding cofactor binding transferase

参与通路

acetyl-CoA biosynthetic process citrate metabolic process oxaloacetate metabolic process fatty acid biosynthetic process cholesterol biosynthetic process lipid biosynthetic process positive regulation of cellular metabolic process long-chain fatty-acyl-CoA biosynthetic process

ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (4H6L2)

ATP Citrate Lyase Antibody (702731) in ICC/IF

产品信息

702731

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀实验 (ChIP)

功能检测 (Functional)

体外检测 (IV)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

Expression System

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

ATP Citrate Lyase Recombinant Rabbit Monoclonal Antibody (4H6L2)

ATP Citrate Lyase Antibody (702731) in ICC/IF

产品信息

702731

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀 实验 (ChIP)

功能检测 (Functional)

体外检测 (IV)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

Expression System

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)