Invitrogen

ATP Synthase gamma Polyclonal Antibody

ATP Synthase gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ATP Synthase gamma Antibody (PA5-29975) in IHC (P)

ATP Synthase gamma Polyclonal Antibody detects ATP synthase gamma protein at mitochondria on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ATP Synthase gamma Polyclonal Antibody (Product # PA5-29975) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

ATP Synthase gamma Antibody in Western Blot (WB)

ATP Synthase gamma Antibody in Immunoprecipitation (IP)

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产品信息

PA5-29975

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 53 and 279 of Human ATP synthase gamma

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.44 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547449

产品详细信息

Recommended positive controls: HeLa, mouse brain, rat kidney.

Predicted reactivity: Mouse (89%), Rat (87%), Bovine (86%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The mitochondrial chaperone TRAP1 regulates F-ATP synthase channel formation.

Cell death and differentiation

Cannino G,Urbani A,Gaspari M,Varano M,Negro A,Filippi A,Ciscato F,Masgras I,Gerle C,Tibaldi E,Brunati AM,Colombo G,Lippe G,Bernardi P,Rasola A

PA5-29975 was used in Western Blotting to identify TRAP1 as an F-ATP synthase regulator that can influence cell bioenergetics and survival and can be targeted in pathological conditions where these processes are dysregulated, such as cancer.

Thu Dec 01 00:00:00 UTC 2022

Toxoplasma gondii GRA8-derived peptide immunotherapy improves tumor targeting of colorectal cancer.

Oncotarget

Kim JS,Lee D,Kim D,Mun SJ,Cho E,Son W,Yang CS

Published figure using ATP Synthase gamma polyclonal antibody (Product # PA5-29975) in Western Blot

Tue Jan 07 00:00:00 UTC 2020

The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore.

Cell reports

Carraro M,Jones K,Sartori G,Schiavone M,Antonucci S,Kucharczyk R,di Rago JP,Franchin C,Arrigoni G,Forte M,Bernardi P

PA5-29975 was used in Western Blotting to investigate the role of C141 of F-ATP synthase oligomycin sensitivity conferral protein (OSCP) subunit in PTP modulation by oxidation.

Tue Sep 01 00:00:00 UTC 2020

Toxoplasma gondii GRA8 induces ATP5A1-SIRT3-mediated mitochondrial metabolic resuscitation: a potential therapy for sepsis.

Experimental & molecular medicine

Kim YR,Kim JS,Yun JS,Kim S,Kim SY,Jang K,Yang CS

PA5-29975 was used in Western Blot to demonstrate a new anti-sepsis therapeutic strategy using Toxoplasma gondii GRA8-induced mitochondrial metabolic resuscitation.

Fri Mar 30 00:00:00 UTC 2018

Toxoplasma gondii GRA8 induces ATP5A1-SIRT3-mediated mitochondrial metabolic resuscitation: a potential therapy for sepsis.

Experimental & molecular medicine

Kim YR,Kim JS,Yun JS,Kim S,Kim SY,Jang K,Yang CS

PA5-29975 was used in Western Blot to demonstrate a new anti-sepsis therapeutic strategy using Toxoplasma gondii GRA8-induced mitochondrial metabolic resuscitation.

Fri Mar 30 00:00:00 UTC 2018

Toxoplasma gondii GRA8 induces ATP5A1-SIRT3-mediated mitochondrial metabolic resuscitation: a potential therapy for sepsis.

Experimental & molecular medicine

Kim YR,Kim JS,Yun JS,Kim S,Kim SY,Jang K,Yang CS

PA5-29975 was used in Western Blot to demonstrate a new anti-sepsis therapeutic strategy using Toxoplasma gondii GRA8-induced mitochondrial metabolic resuscitation.

Fri Mar 30 00:00:00 UTC 2018

生物信息学

蛋白别名: ATP synthase F1 subunit gamma; ATP synthase gamma chain, mitochondrial; ATP synthase gamma-subunit gene encoding a mitochondrial protein; ATP synthase subunit gamma, mitochondrial; F-ATPase gamma subunit; F1 gamma; mitochondrial ATP synthase, gamma subunit 1

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基因别名: 1700094F02Rik; ATP5C; ATP5C1; ATP5CL1; ATP5F1C

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UniProt ID: (Human) P36542, (Mouse) Q91VR2

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Entrez Gene ID: (Human) 509, (Mouse) 11949, (Rat) 116550

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功能

ATPase activity transmembrane transporter activity poly(A) RNA binding proton-transporting ATP synthase activity, rotational mechanism proton-transporting ATPase activity, rotational mechanism ATP synthase

参与通路

oxidative phosphorylation ATP biosynthetic process mitochondrial ATP synthesis coupled proton transport transport ion transport ATP synthesis coupled proton transport proton transport ATP metabolic process