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ATP5G1 Antibody (PA5-103626) in ICC/IF
Immunocytochemistry analysis of ATP5G1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ATP5G1 polyclonal antibody (Product # PA5-103626) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103626
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ATP5G1(Accession P05496), corresponding to amino acid residues A27-V77.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852961
Product Specific Information
Antibody detects endogenous levels of total ATP5G1.
Target Information
Mitochondrial ATP synthase is composed of two multi-subunit complexes that utilize an inner membrane electrochemical gradient to catalyze the synthesis of ATP during oxidative phosphorylation. The two multi-subunit complexes are designated F1 and F0, the former of which comprises the soluble catalytic core and the latter of which comprises the membrane-spanning proton channel of ATP synthase. F1 consists of five distinct subunits, designated ATP5A, ATP5B, ATP5C1, ATP5D and ATP5E, while F0 consists of ten subunits, designated ATP5H, ATP5G1, ATP5I, ATP5G2, ATP5J2, ATP5J, ATP5G3, ATP5S, ATP5F1 and ATP5L. ATP5G1, ATP5G2 and ATP5G3 localize to the mitochondrial membrane and exist as subunits of the F0 complex. ATP5G2 exists as three alternatively spliced isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP Synthase C; ATP synthase F(0) complex subunit C1, mitochondrial; ATP synthase H+ transporting mitochondrial F0 complex subunit c (subunit 9) isoform 1; ATP synthase lipid-binding protein; ATP synthase lipid-binding protein, mitochondrial; ATP synthase membrane subunit c locus 1; ATP synthase proteolipid P1; ATP synthase proton-transporting mitochondrial F(0) complex subunit C1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c, isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1 (subunit 9); ATPase protein 9; ATPase subunit 9; ATPase subunit C; mitochondrial ATP synthase, subunit 9, isoform 1; mitochondrial ATP synthase, subunit C, isoform 1
Gene Aliases: ATP5A; ATP5G; ATP5G1; ATP5MC1
UniProt ID: (Human) P05496, (Mouse) Q9CR84, (Rat) Q06645
Entrez Gene ID: (Human) 516, (Mouse) 11951, (Rat) 29754
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