Invitrogen

ATP6V0A4 Polyclonal Antibody

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ATP6V0A4 Antibody (PA5-96730) in WB

Western blot analysis of ATP6V0A4 in extracts of various cell lines. Samples were incubated with ATP6V0A4 Polyclonal antibody (Product # PA5-96730) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

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产品信息

PA5-96730

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP6V0A4 (NP_0656832)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.16 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2808532

产品详细信息

Immunogen sequence: MVSVFRSEEM CLSQLFLQVE AAYCCVAELG ELGLVQFKDL NMNVNSFQRK FVNEVRRCES LERILRFLED EMQNEIVVQL LEKSPLTPLP REMITLETVL EKLEGELQEA NQNQQALKQS FLELTELKYL LKKTQDFFET ETNLADDFFT EDTSGLLELK AVPAYMTGKL GFIAGVINRE RMASFERLLW RICRGNVYLK FSEMDAPLED PVTKEEIQKN IFIIFYQGEQ LRQKIKKICD GFRATVYPCP EPAVERREML ESVNVRLEDL ITVITQTESH; Positive Samples: A-375, Mouse brain; Cellular Location: Apical cell membrane, Multi-pass membrane protein

靶标信息

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 1B; ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 2 (38kD); ATPase, H+ transporting, lysosomal (vacuolar proton pump) noncatalytic accessory protein 1B; ATPase, H+ transporting, lysosomal V0 subunit a4; H(+)-transporting two-sector ATPase, noncatalytic accessory protein 1B; tcag7.346; V-ATPase 116 kDa; V-ATPase 116 kDa isoform a 4; V-ATPase alpha 4; V-type proton ATPase 116 kDa subunit a; V-type proton ATPase 116 kDa subunit a 4; V-type proton ATPase 116 kDa subunit a isoform 4; vacuol; vacuolar proton pump 116 kDa accessory subunit; vacuolar proton pump, subunit 2; vacuolar proton translocating ATPase 100 kDa a4 subunit; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4; Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform

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基因别名: A4; ATP6N1B; ATP6N2; ATP6V0A4; RDRTA2; RTA1C; RTADR; STV1; VPH1; VPP2

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UniProt ID: (Human) Q9HBG4, (Mouse) Q920B5

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Entrez Gene ID: (Human) 50617, (Mouse) 140494

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功能

protein binding proton-transporting ATPase activity, rotational mechanism ATPase binding hydrogen-exporting ATPase activity, phosphorylative mechanism hydrogen ion transmembrane transporter activity ATP synthase

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