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ATP6V0D1 Antibody (PA5-103179) in ICC/IF
Immunofluorescent analysis of ATP6V0D1 in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ATP6V0D1 polyclonal antibody (Product # PA5-103179) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-103179
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ATP6V0D1(Accession P61421), corresponding to amino acid residues Y261-H311.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2852548
产品详细信息
Antibody detects endogenous levels of total ATP6V0D1.
靶标信息
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 32 kDa accessory protein; ATPase, H+ transporting, lysosomal (vacuolar proton pump), 42 kDa; ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D; ATPase, H+ transporting, lysosomal 38kDa, V0 subunit D; ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1; ATPase, H+ transporting, V0 subunit D; FLJ43534; H(+)-transporting two-sector ATPase, subunit D; lysosomal 38kDa; P39; Physophilin; V-ATPase 40 KDa accessory protein; V-ATPase AC39 subunit; V-ATPase subunit d 1; V-ATPase, subunit D; V-type proton ATPa; V-type proton ATPase subunit d 1; Vacuolar proton pump subunit d 1
基因别名: Ac39; AI267038; ATP6D; ATP6DV; ATP6V0D1; P39; VATX; VMA6; VPATPD
UniProt ID: (Human) P61421, (Mouse) P51863
Entrez Gene ID: (Human) 9114, (Rat) 291969, (Mouse) 11972
brain development
insulin receptor signaling pathway
ATP hydrolysis coupled proton transport
proton transport
regulation of macroautophagy
transferrin transport
ion transmembrane transport
IRE1-mediated unfolded protein response
cilium assembly
phagosome acidification
hydrogen ion transmembrane transport
transport
ion transport
cell projection organization
