Invitrogen

ATP7B Polyclonal Antibody

ATP7B Antibody in Immunocytochemistry (ICC/IF)

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ATP7B Antibody (PA5-143843) in ICC/IF

Immunocytochemistry analysis of ATP7B in Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in ATP7B Polyclonal antibody (Product # PA5-143843) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate... View More

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产品信息

PA5-143843

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human ATP7B recombinant protein (Position: Q241-K495).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3075057

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

靶标信息

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: ATPase, Cu(2+)- transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide (same as Wilson disease); Copper pump 2; Copper-transporting ATPase 2; PINA gene, promoter; pineal night-specific ATPase; RP11-327P2.1; RP11-327P2.3; toxic milk; Wilson disease-associated protein; Wilson disease-associated protein homolog; Wilson protein

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基因别名: Atp7a; ATP7B; Hts; PINA; PWD; tx; WC1; WD; WND

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UniProt ID: (Human) P35670, (Mouse) Q64446

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Entrez Gene ID: (Human) 540, (Rat) 24218, (Mouse) 11979

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