Invitrogen

Acinus Polyclonal Antibody

Acinus Antibody in Immunocytochemistry (ICC/IF)

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Acinus Antibody (PA5-17344) in ICC/IF

Immunofluorescence analysis of ACIN1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Acinus Polyclonal Antibody (Product # PA5-17344) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1:25... View More

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产品信息

PA5-17344

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:1,250

免疫细胞化学 (ICC/IF)

1:50-1:100

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues surrounding Ala835 of Acinus

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

35.1 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10977978

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

ACINUS (apoptotic chromatin condensation inducer in the nucleus) is an induced of chromatin condensation. ACINUS is cleaved by caspase-3 and an additional unknown protease generating a small active peptide, p17, which causes chromatin condensation in vitro when it is added to purified nuclei. ACINUS also induces apoptotic chromatin condensation in cells. ACINUS is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse.

仅用于科研。不用于诊断过程。未经明确授权不得转售。