Invitrogen

Actin Monoclonal Antibody (5J11)

Actin Antibody in Immunocytochemistry (ICC/IF)

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Actin Antibody (MA5-47454) in ICC/IF

Immunofluorescent analysis of Actin in HeLa cells. Samples were stained with Actin monoclonal antibody (Product # MA5-47454) in green. Costained with chicken pAb against vimentin in red. The actin antibody labels the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites. The vimentin antibody stains a different cytoskeletal network, the intermediate or 10 nm filaments. The blue is DAPI staining of nuclear DNA.

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产品信息

MA5-47454

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:500-1:1,000

产品规格

种属反应

Bovine, Horse, Human, Mouse, Pig, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

5J11

抗原

Actin preparation from bovine brain

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS with 50% glycerol

内含物

5mM sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2942436

靶标信息

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: ACTB; actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin 1; alpha actin 2; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; Atcb; B-actin; beta actin; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; cardiac alpha actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; cytoskeletal beta actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; skeletal alpha actin; skeletal muscle alpha-actin 1; smooth muscle alpha-actin; Smooth muscle gamma-actin; vascular smooth muscle alpha-actin

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基因别名: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; ACT-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; ASD5; ASMA; beta-actin; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E430023M04Rik; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMGA; VSCM

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