Invitrogen

Actin Recombinant Rabbit Monoclonal Antibody (JJ09-29)

Actin Antibody in Immunocytochemistry (ICC/IF)

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Actin Antibody (MA5-32479) in ICC/IF

Immunofluorescence analysis of ACTA1 was performed using 70% confluent log phase C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Actin Recombinant Rabbit Monoclonal Antibody (Product # MA5-32479) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 c... View More

Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Actin Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of Actin was observed in Rat Heart, Rat Skeletal Muscle, Mouse Heart and Mouse Skeletal Muscle in comparison to Mouse Kidney and Rat Kidney using Actin Recombinant Rabbit Monoclonal Antibody (Product # MA5-32479) in Western Blot. {RE}

Product Details

MA5-32479

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JJ09-29

Immunogen

Synthetic peptide within Human Actin aa 45-80

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2809756

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epigenetic network of EZH2/SFRP1/Wnt in the epithelial-mesenchymal transition of laryngeal carcinoma cells.

Neoplasma

Chen W,Wang HT,Ji JF,Wang ZY,Shi T,Wu MH,Yu PJ

MA5-32479 was used in Western Blotting to explore the role and mechanism of EZH2 in the epithelial-mesenchymal transition (EMT) of laryngeal carcinoma (LGC) cells.

Sun May 01 00:00:00 UTC 2022

GluR2Q and GluR2R AMPA Subunits are not Targets of lypd2 Interaction.

PloS one

Lauriello A,McVeigh Q,Sung RJ

MA5-32479 was used in Western Blotting to underscore the importance of continuing to investigate novel targets for Ly6 interaction and regulation.

Wed Nov 30 00:00:00 UTC 2022

Co-alterations of circadian clock gene transcripts in human placenta in preeclampsia.

Scientific reports

Zhou G,Winn E,Nguyen D,Kasten EP,Petroff MG,Hoffmann HM

MA5-32479 was used in Western Blotting to provide novel insights into the roles of the molecular clock in the pathogenesis of PE.

Tue Oct 25 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; Atcb; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; sma; smooth muscle alpha-actin; Smooth muscle gamma-actin

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Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; ASD5; ASMA; beta-actin; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E430023M04Rik; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMGA; VSCM; wu:fb63d03