Alomone Labs, LTD

Adenosine A2A Receptor (extracellular) Polyclonal Antibody

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抗体检测数据 (3)

Adenosine A2A Receptor (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))

Adenosine A2A Receptor (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Adenosine A2A Receptor (extracellular) Antibody (AAR-008-200UL) in IHC (F)

Expression ofAdenosineA2AReceptor in rat piriform cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-AdenosineA2AReceptor (extracellular) Antibody (#AAR-008), followed by donkey- Anti-rabbit-biotin and streptavidin-Cy3. AdenosineA2AReceptor immunoreactivity (red), appears in neurons (vertical arrows) and in glial cells in layers 1-2 (horizontal arrows). Cell nuclei ... View More

Adenosine A2A Receptor (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F))

Adenosine A2A Receptor (extracellular) Antibody in Western Blot (WB)

Adenosine A2A Receptor (extracellular) Antibody in Flow Cytometry (Flow)

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Adenosine A2A Receptor (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Expression ofAdenosineA2AReceptor in rat piriform cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-AdenosineA2AReceptor (extracellular) Antibody (#AAR-008), followed by donkey- Anti-rabbit-biotin and streptavidin-Cy3. AdenosineA2AReceptor immunoreactivity (red), appears in neurons (vertical arrows) and in glial cells in layers 1-2 (horizontal arrows). Cell nuclei are stained with DAPI (blue).

Adenosine A2A Receptor (extracellular) Antibody in Western Blot (WB) — Western blot analysis of mouse brain membranes (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and rat spleen membranes (lanes 3 and 6): - 1-3. Anti-AdenosineA2AReceptor (extracellular) Antibody (#AAR-008), (1:200).4-6. Anti-AdenosineA2AReceptor (extracellular) Antibody , preincubated with AdenosineA2AReceptor (extracellular) Blocking Peptide (#BLP-AR008).

Adenosine A2A Receptor (extracellular) Antibody in Flow Cytometry (Flow) — Cell surface detection ofAdenosineA2AReceptor in live intact mouse J774 macrophage cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-AdenosineA2AReceptor (extracellular) Antibody (#AAR-008), (2.5 µg) + goat- Anti-rabbit-FITC.

产品信息

AAR-008-200UL

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

-

免疫组化（冰冻） (IHC (F))

Assay-dependent

-

流式细胞分析 (Flow)

2.5 µg

-

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

(C)SQKDENSTKT(S)GE, corresponding to amino acid residues 143-156 of mouse adenosine A2A receptor, 2nd extracellular loop

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).

靶标信息

ADORA2A is a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. This protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: A2a receptor; Adenosine A2a-receptor; Adenosine receptor A2a; AR; hA2aR

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基因别名: A2AAR; A2aR; AA2AR; ADENO; Adora2a; Adora2l1

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UniProt ID: (Mouse) Q60613, (Rat) P30543

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Entrez Gene ID: (Mouse) 11540, (Rat) 25369

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G-protein coupled adenosine receptor activity signal transducer activity G-protein coupled receptor activity enzyme binding type 5 metabotropic glutamate receptor binding identical protein binding protein heterodimerization activity alpha-actinin binding protein binding G-protein coupled receptor

synaptic transmission, dopaminergic adenosine receptor signaling pathway response to amphetamine regulation of transcription, DNA-templated negative regulation of protein kinase activity signal transduction G-protein coupled receptor signaling pathway adenylate cyclase-activating G-protein coupled receptor signaling pathway protein kinase C-activating G-protein coupled receptor signaling pathway synaptic transmission, cholinergic locomotory behavior negative regulation of cell proliferation positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway positive regulation of glutamate secretion positive regulation of acetylcholine secretion, neurotransmission regulation of norepinephrine secretion positive regulation of cAMP biosynthetic process response to caffeine positive regulation of synaptic transmission, GABAergic synaptic transmission, glutamatergic positive regulation of urine volume positive regulation of renal sodium excretion negative regulation of locomotion vasodilation eating behavior negative regulation of vascular permeability negative regulation of cysteine-type endopeptidase activity involved in apoptotic process response to alkaloid negative regulation of neuron apoptotic process positive regulation of circadian sleep/wake cycle, sleep negative regulation of alpha-beta T cell activation astrocyte activation regulation of synaptic plasticity neuron projection morphogenesis positive regulation of protein secretion negative regulation of inflammatory response regulation of mitochondrial membrane potential membrane depolarization regulation of calcium ion transport positive regulation of synaptic transmission, glutamatergic excitatory postsynaptic potential inhibitory postsynaptic potential prepulse inhibition positive regulation of apoptotic signaling pathway response to drug