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Alomone Labs, LTD
Adenosine A2B Receptor (extracellular) Polyclonal Antibody
FIGURE: 1 / 3
Adenosine A2B Receptor (extracellular) Antibody (AAR-003-200UL) in ICC/IF
Expression of AdenosineA2BReceptorin rat PC12 cells - Cell surface detection of AdenosineA2BReceptor in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-AdenosineA2BReceptor (extracellular) Antibody (#AAR-003), (1:100) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . B. Merge of A and live view of the cells.
Product Details
AAR-003-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KDSATNN*STEPWDGTTNESC, corresponding to amino acid residues 147-166 of humanA2BAR with replacement of cysteine 154 (C154) with serine (*S), 2nd extracellular loop
Conjugate
Form
Lyophilized
Concentration
0.75 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
ADORA2B encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A2b receptor; A2b, Rs; Adenosine receptor A2b; HGNC:264; netrin-1 receptor
Gene Aliases: A2b; A2BAR; A2BR; AA2BR; ADORA2; ADORA2B; AI480866; AI605384
UniProt ID: (Human) P29275, (Rat) P29276, (Mouse) Q60614
Entrez Gene ID: (Human) 136, (Rat) 29316, (Mouse) 11541
activation of MAPK activity
adenosine receptor signaling pathway
positive regulation of chronic inflammatory response to non-antigenic stimulus
cellular defense response
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
activation of adenylate cyclase activity
JNK cascade
excretion
positive regulation of vascular endothelial growth factor production
positive regulation of guanylate cyclase activity
cellular response to extracellular stimulus
positive regulation of chemokine production
positive regulation of interleukin-6 production
positive regulation of mast cell degranulation
cellular protein metabolic process
relaxation of vascular smooth muscle
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of norepinephrine secretion
positive regulation of steroid biosynthetic process
positive regulation of cAMP biosynthetic process
positive regulation of cGMP biosynthetic process
negative regulation of collagen biosynthetic process
positive regulation of catecholamine secretion
regulation of angiogenesis
positive regulation of vasodilation
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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