Invitrogen

Adenosine Deaminase Recombinant Rabbit Monoclonal Antibody (ARC1152)

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抗体检测数据 (2)

Adenosine Deaminase Antibody in Western Blot (WB)

Adenosine Deaminase Antibody in Western Blot (WB)

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Adenosine Deaminase Antibody (MA5-38137) in WB

Western blot analysis of Adenosine Deaminase in various lysates. Samples were incubated with Adenosine Deaminase Monoclonal antibody (Product # MA5-38137) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Adenosine Deaminase Antibody in Western Blot (WB)

Adenosine Deaminase Antibody in Western Blot (WB)

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Adenosine Deaminase Antibody in Western Blot (WB) — Western blot analysis of Adenosine Deaminase in various lysates. Samples were incubated with Adenosine Deaminase Monoclonal antibody (Product # MA5-38137) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Adenosine Deaminase Antibody in Western Blot (WB) — Western blot analysis of Adenosine Deaminase in mouse stomach. Samples were incubated with Adenosine Deaminase Monoclonal antibody (Product # MA5-38137) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.

产品信息

MA5-38137

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

ARC1152

抗原

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Adenosine Deaminase (ADA) (NP_0000132)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2898055

产品详细信息

Positive Samples: Jurkat, Mouse stomach

Immunogen sequence: NRLRQENMHF EICPWSSYLT GAWKPDTEHA VIRLKNDQAN YSLNTDDPLI FKSTLDTDYQ MTKRDMGFTE EEFKRLNINA AKSSFLPEDE KRELLDLLYK A

靶标信息

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. May act as a positive regulator of T-cell coactivation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Adenosine aminohydrolase; Adenosine deaminase; RP11-61L14.5

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基因别名: ADA; ADA1

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UniProt ID: (Human) P00813, (Mouse) P03958

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Entrez Gene ID: (Human) 100, (Mouse) 11486

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purine nucleoside binding adenosine deaminase activity protein binding zinc ion binding hydrolase activity deaminase activity metal ion binding deaminase

response to hypoxia histamine secretion trophectodermal cell differentiation liver development placenta development germinal center B cell differentiation positive regulation of germinal center formation negative regulation of leukocyte migration negative regulation of mature B cell apoptotic process adenosine catabolic process deoxyadenosine catabolic process aging purine ribonucleoside monophosphate biosynthetic process positive regulation of heart rate positive regulation of B cell proliferation purine nucleotide salvage positive regulation of T cell differentiation in thymus response to vitamin E regulation of cell-cell adhesion mediated by integrin T cell activation negative regulation of circadian sleep/wake cycle, non-REM sleep response to drug response to hydrogen peroxide purine-containing compound salvage hypoxanthine salvage response to morphine positive regulation of smooth muscle contraction dATP catabolic process inosine biosynthetic process xanthine biosynthetic process positive regulation of alpha-beta T cell differentiation lung alveolus development Peyer's patch development embryonic digestive tract development negative regulation of inflammatory response positive regulation of calcium-mediated signaling positive regulation of T cell receptor signaling pathway negative regulation of adenosine receptor signaling pathway negative regulation of penile erection negative regulation of thymocyte apoptotic process negative regulation of mucus secretion in utero embryonic development cell adhesion nucleotide metabolic process lung development negative regulation of apoptotic process regulation of T cell differentiation positive regulation of T cell differentiation adenosine metabolic process hypoxanthine biosynthetic process positive regulation of T cell activation